AlanFung:LabNotes/CTCF/2010-4-8: Difference between revisions
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*2ul sample + 3ul ddh2o + 5ul 2X TBU Buffer | *2ul sample + 3ul ddh2o + 5ul 2X TBU Buffer | ||
[[File:ZhangLab_2 2010-04-08 12hr 46min.jpg]] | [[File:ZhangLab_2 2010-04-08 12hr 46min.jpg]] | ||
*By comparing the gel image from the TBE Gel Quantification on 3/24/10, it appears that the TBE gel matrix hinders the migration of the double stranded library and gives a false size of the DNA, while the TBU gel shows that that the band at 100BP (original DNA size) 150BP and 180BP. | *By comparing the gel image from the TBE Gel Quantification on 3/24/10, it appears that the TBE gel matrix hinders the migration of the double stranded library and gives a false size of the DNA, while the TBU gel shows that that the band at 100BP (original DNA size) 150BP and 180BP. I was expecting a library at around 130 BP when the library is denatured, for the PE adapter is 32 and 33bp. |
Revision as of 21:46, 8 April 2010
Denaturing Gel Quantification
- 0.5ul low mass ladder + 4.5ul ddh2o + 5ul 2x TBU Buffer
- 2ul sample + 3ul ddh2o + 5ul 2X TBU Buffer
File:ZhangLab 2 2010-04-08 12hr 46min.jpg
- By comparing the gel image from the TBE Gel Quantification on 3/24/10, it appears that the TBE gel matrix hinders the migration of the double stranded library and gives a false size of the DNA, while the TBU gel shows that that the band at 100BP (original DNA size) 150BP and 180BP. I was expecting a library at around 130 BP when the library is denatured, for the PE adapter is 32 and 33bp.