AlanFung:LabNotes/CTCF/2010-4-8: Difference between revisions

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*2ul sample + 3ul ddh2o + 5ul 2X TBU Buffer
*2ul sample + 3ul ddh2o + 5ul 2X TBU Buffer
[[File:ZhangLab_2 2010-04-08 12hr 46min.jpg]]
[[File:ZhangLab_2 2010-04-08 12hr 46min.jpg]]
*By comparing the gel image from the TBE Gel Quantification on 3/24/10, it appears that the TBE gel matrix hinders the migration of the double stranded library and gives a false size of the DNA, while the TBU gel shows that that the band at 100BP (original DNA size) 150BP and 180BP. I was expecting a library at around 130 BP when the library is denatured, for the PE adapter is 32 and 33bp.
*By comparing the gel image from the TBE Gel Quantification on 3/24/10, it appears that the TBE gel matrix hinders the migration of the double stranded library and gives a false size of the DNA, while the TBU gel shows that that the band at 100BP (original DNA size) 150BP and 180BP. It appears that we still have some half ligated and non ligated product.

Latest revision as of 16:35, 9 April 2010

Denaturing Gel Quantification[edit]

  • 0.5ul low mass ladder + 4.5ul ddh2o + 5ul 2x TBU Buffer
  • 2ul sample + 3ul ddh2o + 5ul 2X TBU Buffer

File:ZhangLab 2 2010-04-08 12hr 46min.jpg

  • By comparing the gel image from the TBE Gel Quantification on 3/24/10, it appears that the TBE gel matrix hinders the migration of the double stranded library and gives a false size of the DNA, while the TBU gel shows that that the band at 100BP (original DNA size) 150BP and 180BP. It appears that we still have some half ligated and non ligated product.