PrepGEM (ZyGEM) Preparation: Difference between revisions
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>Jbrubake (Created page with '=Preparation= Preparing the PrepGEM denaturant is very simple: Per reaction: - 1uL 10X Gold Buffer - 0.9uL water - 0.1uL PrepGEM substrate') |
>Jbrubake |
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Line 7: | Line 7: | ||
- 0.9uL water | - 0.9uL water | ||
- 0.1uL PrepGEM substrate | - 0.1uL PrepGEM substrate | ||
---Revised (as of 4/30/10)--- | |||
Per reaction: | |||
- 0.1uL 10X Gold Buffer | |||
- 0.89uL water | |||
- 0.01uL PrepGEM substrate | |||
'''Note:''' In order to make a reasonable mixture, enough volume for at least 10 reactions should be mixed at once and split up as much as needed. |
Latest revision as of 20:52, 3 May 2010
Preparation[edit]
Preparing the PrepGEM denaturant is very simple:
Per reaction: - 1uL 10X Gold Buffer - 0.9uL water - 0.1uL PrepGEM substrate
---Revised (as of 4/30/10)---
Per reaction: - 0.1uL 10X Gold Buffer - 0.89uL water - 0.01uL PrepGEM substrate
Note: In order to make a reasonable mixture, enough volume for at least 10 reactions should be mixed at once and split up as much as needed.