Sam:LabNotes/Microbiome-new/2010-9-24: Difference between revisions
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>Dinh (Created page with '='''Sanger sequencing test on E.coli gDNA MDA amplicons'''= ==Background==') |
>Sam Chiang No edit summary |
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==Background== | ==Background== | ||
*To optimize the Sanger sequencing protocl for MDA amplicon validation | |||
*This protocol will also include the S1 nucelase debranching, DNA Pol.I reparing procedures, and compare the difference between with and without these treatments. | |||
==Proceduress== |
Revision as of 15:29, 25 September 2010
Sanger sequencing test on E.coli gDNA MDA amplicons
Background
- To optimize the Sanger sequencing protocl for MDA amplicon validation
- This protocol will also include the S1 nucelase debranching, DNA Pol.I reparing procedures, and compare the difference between with and without these treatments.