Sam:LabNotes/Microbiome-new/2010-9-24: Difference between revisions

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>Dinh
(Created page with '='''Sanger sequencing test on E.coli gDNA MDA amplicons'''= ==Background==')
 
>Sam Chiang
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==Background==
==Background==
*To optimize the Sanger sequencing protocl for MDA amplicon validation
*This protocol will also include the S1 nucelase debranching, DNA Pol.I reparing procedures, and compare the difference between with and without these treatments.
==Proceduress==

Revision as of 15:29, 25 September 2010

Sanger sequencing test on E.coli gDNA MDA amplicons

Background

  • To optimize the Sanger sequencing protocl for MDA amplicon validation
  • This protocol will also include the S1 nucelase debranching, DNA Pol.I reparing procedures, and compare the difference between with and without these treatments.

Proceduress