AlanFung:LabNotes/Reprogramming/2010-11-10: Difference between revisions

From ZhangLabWiki
Jump to navigation Jump to search
>Alan6017518
>Alan6017518
Line 1: Line 1:
==one step qRT-PCR==
==one step qRT-PCR==
*The purpose of this experiment is to perform quantitative RT-PCR for the detection of the efficiency of SPEN gene knockdown.
*The purpose of this experiment is to perform quantitative RT-PCR for the detection of the efficiency of SPEN gene knockdown.
Running at Program “PCR62” (Tm=62, 30cycle)
*Running at Program “one step qrt_pcr"
50C 3min -> hold -> 95C 5min -> hold (95C 15sec -> 60C 30sec) x 40 cycle -> 40C 1min
*50C 3min -> hold -> 95C 5min -> hold (95C 15sec -> 60C 30sec) x 40 cycle -> 40C 1min

Revision as of 23:16, 9 November 2010

one step qRT-PCR

  • The purpose of this experiment is to perform quantitative RT-PCR for the detection of the efficiency of SPEN gene knockdown.
  • Running at Program “one step qrt_pcr"
  • 50C 3min -> hold -> 95C 5min -> hold (95C 15sec -> 60C 30sec) x 40 cycle -> 40C 1min