Rui Liu: Difference between revisions
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===Ep Realplex QPCR machine=== | ===Ep Realplex QPCR machine=== | ||
===Microscope=== |
Revision as of 18:17, 8 February 2011
Lab Projects
Lab Meetings
Manuals
Bio-Rad QPCR machine
- Program on desktop: "Opticon Moniter 3"
- Instrument: Chromo4:CD003042. "Instrument"-->"Scan for instruments" or "Quick load"(under "Master")-->choose "Chromo4:CD003042"
- "Prepare new run" or "open" a old file and "edit"
- "Plate setup"-->"edit"-->select "samples" and "SBG1" for SYBR green I
- "Protocol setup"-->"edit"-->add "plate read" before "repeat cycle"
- "Melting curve" optional, hold at 15C, volume 20ul
- "Run" and "Save", files are saved in "Opticon Users 3"/Rui
- "Status" and "Quantitation" are used for monitoring PCR process
- "Quantitation"-->"copy to clipboard"-->"Data graph" and edit under "Paint"