Rui:LabNotes/SingleCell/2011-1-26: Difference between revisions

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>RuiLiu
(Created page with '==Trouble shooting for PCR==')
 
>RuiLiu
Line 1: Line 1:
==Trouble shooting for PCR==
==Trouble shooting for PCR==
'''Components in tubes'''
----------------------------------------------------------------------------------
ID Template      P1  P2  others  Taq SYBR
----------------------------------------------------------------------------------
Tube1 Rui water    Yes  Yes   Yes  Yes           
Tube2  Kun water    Yes  Yes    Yes  Yes           
Tube3  Sam water    Yes  Yes          Yes  Yes
Tube4  10pg RNA    Yes  Yes          Yes  Yes
Tube5  Rui water    Yes                Yes  Yes
Tube6  Rui water          Yes          Yes  Yes
Tube7  Rui water              P3+P4    Yes  Yes
Tube8  Rui water              P3+p5    Yes  Yes
Tube9  Rui water                              Yes
Tube10 Rui water                        Yes                                 
----------------------------------------------------------------------------------
Common components: Ex Taq buffer, dNTP mix
Aim 1: check contamination in Rui water; 10pg RNA sample
Aim 2: check primer hetero-dimer
SYBR(-) and Taq(-) are negative control

Revision as of 20:51, 26 January 2011

Trouble shooting for PCR

Components in tubes
----------------------------------------------------------------------------------
ID	 Template      P1   P2   others   Taq	SYBR
---------------------------------------------------------------------------------- 
Tube1	 Rui water    Yes   Yes 	  Yes   Yes            
Tube2   Kun water    Yes   Yes  	  Yes   Yes     	        
Tube3   Sam water    Yes   Yes           Yes   Yes
Tube4   10pg RNA     Yes   Yes           Yes   Yes
Tube5   Rui water    Yes                 Yes   Yes
Tube6   Rui water          Yes           Yes   Yes
Tube7   Rui water               P3+P4    Yes   Yes
Tube8   Rui water               P3+p5    Yes   Yes
Tube9   Rui water                              Yes
Tube10	 Rui water                        Yes   	                         	         
----------------------------------------------------------------------------------
Common components: Ex Taq buffer, dNTP mix
Aim 1: check contamination in Rui water; 10pg RNA sample
Aim 2: check primer hetero-dimer
SYBR(-) and Taq(-) are negative control