Rui:LabNotes/SingleCell/2011-1-28: Difference between revisions
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>RuiLiu (Created page with '==Titration Exonuclease I (continued)== *Each samples (tube1 to 8) have 4 aliquots for PCR, 40ul/aliquotes *4ul PCR products from one aliquot per sample was checked by 6% TBE-…') |
>RuiLiu |
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*Each samples (tube1 to 8) have 4 aliquots for PCR, 40ul/aliquotes | *Each samples (tube1 to 8) have 4 aliquots for PCR, 40ul/aliquotes | ||
*4ul PCR products from one aliquot per sample was checked by 6% TBE-PAGE | *4ul PCR products from one aliquot per sample was checked by 6% TBE-PAGE | ||
* | |||
'''Sample arrangement''' | |||
Template P1 primer SSIII Exo buffer ExoI Final | |||
Tube1 water 0.125ul 0.33ul 1* in 1ul 0.5U 0.16* in 6ul | |||
Tube2 water 0.125ul 0.33ul 1* in 1ul 2U 0.16* in 6ul | |||
Tube3 water 0.125ul 0.33ul 3* in 1ul 4U 0.5* in 6ul | |||
Tube4 water 0.125ul 0.33ul 6* in 1ul 8U 1* in 6ul | |||
Tube5 0.1pg 6-EB cDNA 1* in 1ul 0.5U 0.16* in 6ul | |||
Tube6 0.1pg 6-EB cDNA 1* in 1ul 2U 0.16* in 6ul | |||
Tube7 0.1pg 6-EB cDNA 3* in 1ul 4U 0.5* in 6ul | |||
Tube8 0.1pg 6-EB cDNA 6* in 1ul 8U 1* in 6ul |
Revision as of 20:47, 28 January 2011
Titration Exonuclease I (continued)
*Each samples (tube1 to 8) have 4 aliquots for PCR, 40ul/aliquotes *4ul PCR products from one aliquot per sample was checked by 6% TBE-PAGE Sample arrangement Template P1 primer SSIII Exo buffer ExoI Final Tube1 water 0.125ul 0.33ul 1* in 1ul 0.5U 0.16* in 6ul Tube2 water 0.125ul 0.33ul 1* in 1ul 2U 0.16* in 6ul Tube3 water 0.125ul 0.33ul 3* in 1ul 4U 0.5* in 6ul Tube4 water 0.125ul 0.33ul 6* in 1ul 8U 1* in 6ul Tube5 0.1pg 6-EB cDNA 1* in 1ul 0.5U 0.16* in 6ul Tube6 0.1pg 6-EB cDNA 1* in 1ul 2U 0.16* in 6ul Tube7 0.1pg 6-EB cDNA 3* in 1ul 4U 0.5* in 6ul Tube8 0.1pg 6-EB cDNA 6* in 1ul 8U 1* in 6ul