Rui:LabNotes/SingleCell/2011-1-28: Difference between revisions

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>RuiLiu
(Created page with '==Titration Exonuclease I (continued)== *Each samples (tube1 to 8) have 4 aliquots for PCR, 40ul/aliquotes *4ul PCR products from one aliquot per sample was checked by 6% TBE-…')
 
>RuiLiu
Line 3: Line 3:
  *Each samples (tube1 to 8) have 4 aliquots for PCR, 40ul/aliquotes
  *Each samples (tube1 to 8) have 4 aliquots for PCR, 40ul/aliquotes
  *4ul PCR products from one aliquot per sample was checked by 6% TBE-PAGE  
  *4ul PCR products from one aliquot per sample was checked by 6% TBE-PAGE  
  *
   
'''Sample arrangement'''
        Template        P1 primer  SSIII    Exo buffer    ExoI      Final
Tube1  water            0.125ul    0.33ul  1* in 1ul    0.5U      0.16* in 6ul
Tube2  water            0.125ul    0.33ul  1* in 1ul    2U        0.16* in 6ul
Tube3  water            0.125ul    0.33ul  3* in 1ul    4U        0.5* in 6ul
Tube4  water            0.125ul    0.33ul  6* in 1ul    8U        1* in 6ul
Tube5  0.1pg 6-EB cDNA                      1* in 1ul    0.5U      0.16* in 6ul     
Tube6  0.1pg 6-EB cDNA                      1* in 1ul    2U        0.16* in 6ul
Tube7  0.1pg 6-EB cDNA                      3* in 1ul    4U        0.5* in 6ul
Tube8  0.1pg 6-EB cDNA                      6* in 1ul    8U        1* in 6ul

Revision as of 20:47, 28 January 2011

Titration Exonuclease I (continued)

*Each samples (tube1 to 8) have 4 aliquots for PCR, 40ul/aliquotes
*4ul PCR products from one aliquot per sample was checked by 6% TBE-PAGE 

Sample arrangement 
        Template         P1 primer   SSIII    Exo buffer    ExoI       Final
Tube1   water            0.125ul     0.33ul   1* in 1ul     0.5U       0.16* in 6ul
Tube2   water            0.125ul     0.33ul   1* in 1ul     2U         0.16* in 6ul
Tube3   water            0.125ul     0.33ul   3* in 1ul     4U         0.5* in 6ul
Tube4   water            0.125ul     0.33ul   6* in 1ul     8U         1* in 6ul
Tube5   0.1pg 6-EB cDNA                       1* in 1ul     0.5U       0.16* in 6ul       
Tube6   0.1pg 6-EB cDNA                       1* in 1ul     2U         0.16* in 6ul
Tube7   0.1pg 6-EB cDNA                       3* in 1ul     4U         0.5* in 6ul
Tube8   0.1pg 6-EB cDNA                       6* in 1ul     8U         1* in 6ul