AlanFung:LabNotes/CTCF/2011-2-14: Difference between revisions

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(Created page with '==Protocol== *Nuclease Free water 100ng input DNA 4ul 5x Nextera HMW Reaction Buffer 1ul Nextera Methyl-Seq Tagmentation Mix')
 
>Alan6017518
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='''Nextera Methyl-Seq DNA Sample Prep'''=
==Objective==
*Confirmation of the library prep with beta nextera methyl-seq kit with input DNA (100ng starting amount) and size around 200-1000BP
==Protocol==
==Protocol==
*Nuclease Free water
*Nuclease Free water

Revision as of 01:17, 15 February 2011

Nextera Methyl-Seq DNA Sample Prep

Objective

  • Confirmation of the library prep with beta nextera methyl-seq kit with input DNA (100ng starting amount) and size around 200-1000BP

Protocol

  • Nuclease Free water
100ng input DNA 
4ul 5x Nextera HMW Reaction Buffer
1ul Nextera Methyl-Seq Tagmentation Mix