AlanFung:LabNotes/CTCF/2011-2-14: Difference between revisions
Jump to navigation
Jump to search
>Alan6017518 |
>Alan6017518 |
||
Line 11: | Line 11: | ||
*Qiagen Purification Kit | *Qiagen Purification Kit | ||
==Overview== | ==Overview== | ||
*Tagmentation | |||
*Purification | |||
*Fill-in | |||
*Purification | |||
*Bisulfite Conversion | |||
*Purification | |||
*bPCR-compatible sites and library enrichment | |||
==Protocol== | ==Protocol== |
Revision as of 01:22, 15 February 2011
Nextera Methyl-Seq DNA Sample Prep
Objective
- Confirmation of the library prep with beta nextera methyl-seq kit with input DNA (100ng starting amount) and size around 200-1000BP
Samples & Materials
- Nextera Sample Prep Kit_Epicentre_NMS072910B
- Input DNA
- Bisulfite conversion kit
- Qiagen Purification Kit
Overview
- Tagmentation
- Purification
- Fill-in
- Purification
- Bisulfite Conversion
- Purification
- bPCR-compatible sites and library enrichment
Protocol
- Nuclease Free water
100ng input DNA 4ul 5x Nextera HMW Reaction Buffer 1ul Nextera Methyl-Seq Tagmentation Mix