AlanFung:LabNotes/CTCF/2011-2-14: Difference between revisions
Jump to navigation
Jump to search
>Alan6017518 |
>Alan6017518 |
||
Line 7: | Line 7: | ||
==Samples & Materials== | ==Samples & Materials== | ||
*Nextera Sample Prep Kit_Epicentre_NMS072910B | *Nextera Sample Prep Kit_Epicentre_NMS072910B | ||
NexteraTM Methyl-Seq Tagmentation Mix (Illumina-compatible) | |||
5X Nextera Reaction Buffer (HMW) | |||
50X Nextera Primer Cocktail (Illumina-compatible) | |||
50X Nextera Adaptor 2 (Illumina-compatible) | |||
2X Nextera Fill-in Buffer | |||
2X Nextera PCR Buffer | |||
200X Nextera Read 1 Primer | |||
200X Nextera Read 2 Primer | |||
200X Nextera Index Read Primer | |||
Nextera Methyl-Seq Polymerase | |||
*Input DNA | *Input DNA | ||
*Bisulfite conversion kit | *Bisulfite conversion kit | ||
*Qiagen Purification Kit | *Qiagen Purification Kit | ||
==Overview== | ==Overview== | ||
*Tagmentation | *Tagmentation |
Revision as of 01:31, 15 February 2011
Nextera Methyl-Seq DNA Sample Prep
Objective
- Confirmation of the library prep with beta nextera methyl-seq kit with input DNA (100ng starting amount) and size around 200-1000BP
Samples & Materials
- Nextera Sample Prep Kit_Epicentre_NMS072910B
NexteraTM Methyl-Seq Tagmentation Mix (Illumina-compatible) 5X Nextera Reaction Buffer (HMW) 50X Nextera Primer Cocktail (Illumina-compatible) 50X Nextera Adaptor 2 (Illumina-compatible) 2X Nextera Fill-in Buffer 2X Nextera PCR Buffer 200X Nextera Read 1 Primer 200X Nextera Read 2 Primer 200X Nextera Index Read Primer Nextera Methyl-Seq Polymerase
- Input DNA
- Bisulfite conversion kit
- Qiagen Purification Kit
Overview
- Tagmentation
- Purification
- Fill-in
- Purification
- Bisulfite Conversion
- Purification
- bPCR-compatible sites and library enrichment
Protocol
- Nuclease Free water
100ng input DNA 4ul 5x Nextera HMW Reaction Buffer 1ul Nextera Methyl-Seq Tagmentation Mix