Athurva Gore/LabNotes/2011-4-8: Difference between revisions

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* '''Can we modify the experiment to still allow attachment to beads?'''
* '''Can we modify the experiment to still allow attachment to beads?'''
** According to Invitrogen website [http://www.invitrogen.com/site/us/en/home/brands/Dynal/Streptavidin-Coupled-Dynabeads/dynabeads_streptavidin.html#How%20do%20I%20dissociate%20my%20biotinylated%20molecule], bead-detachment can occur when:
** According to Invitrogen website [http://www.invitrogen.com/site/us/en/home/brands/Dynal/Streptavidin-Coupled-Dynabeads/dynabeads_streptavidin.html#How%20do%20I%20dissociate%20my%20biotinylated%20molecule], bead-detachment can occur when:
*** fds
*** Heat in formamide or basic solution at 95 for 2 min or 65 for 5 min
*** Boil beads

Revision as of 21:59, 8 April 2011

Bisulfite Biotin-Binding

  • Ran gel for Bisulfite-converted bead-bound DNA and normal bead-bound DNA:

File:04072011 Low Input Bisulfite FAILED.jpg

  • Bisulfite-converted libraries did not work.
    • DNA was definitely attached to beads, as normal amplification worked.
    • DNA must have been detached
  • Can we modify the experiment to still allow attachment to beads?
    • According to Invitrogen website [1], bead-detachment can occur when:
      • Heat in formamide or basic solution at 95 for 2 min or 65 for 5 min
      • Boil beads