Athurva Gore/LabNotes/2011-4-8: Difference between revisions
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* '''Can we modify the experiment to still allow attachment to beads?''' | * '''Can we modify the experiment to still allow attachment to beads?''' | ||
** According to Invitrogen website [http://www.invitrogen.com/site/us/en/home/brands/Dynal/Streptavidin-Coupled-Dynabeads/dynabeads_streptavidin.html#How%20do%20I%20dissociate%20my%20biotinylated%20molecule], bead-detachment can occur when: | ** According to Invitrogen website [http://www.invitrogen.com/site/us/en/home/brands/Dynal/Streptavidin-Coupled-Dynabeads/dynabeads_streptavidin.html#How%20do%20I%20dissociate%20my%20biotinylated%20molecule], bead-detachment can occur when: | ||
*** | *** Heat in formamide or basic solution at 95 for 2 min or 65 for 5 min | ||
*** Boil beads |
Revision as of 21:59, 8 April 2011
Bisulfite Biotin-Binding
- Ran gel for Bisulfite-converted bead-bound DNA and normal bead-bound DNA:
File:04072011 Low Input Bisulfite FAILED.jpg
- Bisulfite-converted libraries did not work.
- DNA was definitely attached to beads, as normal amplification worked.
- DNA must have been detached
- Can we modify the experiment to still allow attachment to beads?
- According to Invitrogen website [1], bead-detachment can occur when:
- Heat in formamide or basic solution at 95 for 2 min or 65 for 5 min
- Boil beads
- According to Invitrogen website [1], bead-detachment can occur when: