Sam:LabNotes/Microbiome-new/2011-4-27: Difference between revisions

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>Sam Chiang
(Created page with '='''Mouse gut bacteria SAG ("c5")preparation'''= ==Objective== *I prepared two Illumina libraries using limited MDA (2hr incubation) strategy, but only one of the samples was se…')
 
>Sam Chiang
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==Illumina library amplification references==
==Illumina library amplification references==
*http://genome-tech.ucsd.edu/LabNotes/index.php/Sam:LabNotes/Microbiome-new/2010-11-07_exp1
*Ligation product amplification with N2-indexed primer
*http://genome-tech.ucsd.edu/LabNotes/index.php/Sam:LabNotes/Microbiome-new/2010-11-14
**http://genome-tech.ucsd.edu/LabNotes/index.php/Sam:LabNotes/Microbiome-new/2010-11-07_exp1
*http://genome-tech.ucsd.edu/LabNotes/index.php/Sam:LabNotes/Microbiome-new/2010-11-15
*AMpure bead purification
**http://genome-tech.ucsd.edu/LabNotes/index.php/Sam:LabNotes/Microbiome-new/2010-11-14
*2nd size-selection
http://genome-tech.ucsd.edu/LabNotes/index.php/Sam:LabNotes/Microbiome-new/2010-11-15

Revision as of 00:46, 28 April 2011

Mouse gut bacteria SAG ("c5")preparation

Objective

  • I prepared two Illumina libraries using limited MDA (2hr incubation) strategy, but only one of the samples was sequenced.


Illumina library amplification references

http://genome-tech.ucsd.edu/LabNotes/index.php/Sam:LabNotes/Microbiome-new/2010-11-15