Rui:LabNotes/SingleCell/2011-5-20: Difference between revisions

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==Beads purification==
==Repeat 2nd strands and PCR with the remaining samples==
{| {{table}}
* Continue with samples on 5.19.11, the remaining 9ul A-tailing product
| align="center" style="background:#f0f0f0;"|'''Sample ID'''
* 2nd strand with P8
| align="center" style="background:#f0f0f0;"|'''ng/ul'''
* PCR with 30 cycles
| align="center" style="background:#f0f0f0;"|'''260/280'''
| align="center" style="background:#f0f0f0;"|'''260/230'''
|-
| sperm RNA||43.64||1.9||1.68
|-
| ES RNA||295.67||1.93||2.18
|-
| ES lysis||220.08||1.92||1.97
|-
| sperm lysis||23.5||1.68||0.95
|-
|
|}

Revision as of 17:14, 23 May 2011


Additional 10 cycles of direct lysis samples

  • Continue with lab note on 5.19.11
  • Additional 10 cycles for direct lysis samples (sperm and ES) in parallel with 500pg RNA and 150pg gDNA

QPCR curve

File:Additional 10 cycles 5.20.11.jpg

TBE PAGE

File:10 cycles 5.20.11.jpg


Repeat 2nd strands and PCR with the remaining samples

  • Continue with samples on 5.19.11, the remaining 9ul A-tailing product
  • 2nd strand with P8
  • PCR with 30 cycles