Rui:LabNotes/SingleCell/2011-5-23: Difference between revisions
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>RuiLiu (Created page with '==Library construct by Nextera== ==Library construct by ligation with Y adaptor== ==Cloning of PCR product==') |
>RuiLiu |
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==Library construct by ligation with Y adaptor== | ==Library construct by ligation with Y adaptor== | ||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''>Digestion''' | |||
| align="center" style="background:#f0f0f0;"|'''ng/ul''' | |||
| align="center" style="background:#f0f0f0;"|'''260/280''' | |||
| align="center" style="background:#f0f0f0;"|'''260/230''' | |||
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| sperm RNA||39.12||1.9||2.77 | |||
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| ES RNA||51.57||1.92||1.92 | |||
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| sperm lysis||16.27||1.73||1.42 | |||
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| ES lysis||45.64||1.93||1.99 | |||
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| >Ligation||ng/ul ||260/280 ||260/230 | |||
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| sperm RNA||35.03||2.05||2.19 | |||
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| ES RNA||45.66||1.92||1.99 | |||
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| sperm lysis||13.99||2.04||1.7 | |||
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| ES lysis||39.23||2.06||2.12 | |||
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| >PCR||ng/ul ||260/280 ||260/230 | |||
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| sperm RNA ID5||21.29||1.73||1.69 | |||
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| ES RNA ID6||21.31||1.72||1.7 | |||
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| sperm lysis ID7||19.24||1.61||1.14 | |||
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| ES lysis ID8||20.03||1.74||1.1 | |||
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==Cloning of PCR product== | ==Cloning of PCR product== |
Revision as of 19:27, 24 May 2011
Library construct by Nextera
Library construct by ligation with Y adaptor
>Digestion | ng/ul | 260/280 | 260/230 |
sperm RNA | 39.12 | 1.9 | 2.77 |
ES RNA | 51.57 | 1.92 | 1.92 |
sperm lysis | 16.27 | 1.73 | 1.42 |
ES lysis | 45.64 | 1.93 | 1.99 |
>Ligation | ng/ul | 260/280 | 260/230 |
sperm RNA | 35.03 | 2.05 | 2.19 |
ES RNA | 45.66 | 1.92 | 1.99 |
sperm lysis | 13.99 | 2.04 | 1.7 |
ES lysis | 39.23 | 2.06 | 2.12 |
>PCR | ng/ul | 260/280 | 260/230 |
sperm RNA ID5 | 21.29 | 1.73 | 1.69 |
ES RNA ID6 | 21.31 | 1.72 | 1.7 |
sperm lysis ID7 | 19.24 | 1.61 | 1.14 |
ES lysis ID8 | 20.03 | 1.74 | 1.1 |