Rui:LabNotes/SingleCell/2011-5-23: Difference between revisions

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>RuiLiu
(Created page with '==Library construct by Nextera== ==Library construct by ligation with Y adaptor== ==Cloning of PCR product==')
 
>RuiLiu
Line 2: Line 2:


==Library construct by ligation with Y adaptor==
==Library construct by ligation with Y adaptor==
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''>Digestion'''
| align="center" style="background:#f0f0f0;"|'''ng/ul'''
| align="center" style="background:#f0f0f0;"|'''260/280'''
| align="center" style="background:#f0f0f0;"|'''260/230'''
|-
| sperm RNA||39.12||1.9||2.77
|-
| ES RNA||51.57||1.92||1.92
|-
| sperm lysis||16.27||1.73||1.42
|-
| ES lysis||45.64||1.93||1.99
|-
| ||||||
|-
| ||||||
|-
| >Ligation||ng/ul ||260/280 ||260/230
|-
| sperm RNA||35.03||2.05||2.19
|-
| ES RNA||45.66||1.92||1.99
|-
| sperm lysis||13.99||2.04||1.7
|-
| ES lysis||39.23||2.06||2.12
|-
| ||||||
|-
| ||||||
|-
| >PCR||ng/ul ||260/280 ||260/230
|-
| sperm RNA ID5||21.29||1.73||1.69
|-
| ES RNA ID6||21.31||1.72||1.7
|-
| sperm lysis ID7||19.24||1.61||1.14
|-
| ES lysis ID8||20.03||1.74||1.1
|-
|
|}


==Cloning of PCR product==
==Cloning of PCR product==

Revision as of 19:27, 24 May 2011

Library construct by Nextera

Library construct by ligation with Y adaptor

>Digestion ng/ul 260/280 260/230
sperm RNA 39.12 1.9 2.77
ES RNA 51.57 1.92 1.92
sperm lysis 16.27 1.73 1.42
ES lysis 45.64 1.93 1.99
>Ligation ng/ul 260/280 260/230
sperm RNA 35.03 2.05 2.19
ES RNA 45.66 1.92 1.99
sperm lysis 13.99 2.04 1.7
ES lysis 39.23 2.06 2.12
>PCR ng/ul 260/280 260/230
sperm RNA ID5 21.29 1.73 1.69
ES RNA ID6 21.31 1.72 1.7
sperm lysis ID7 19.24 1.61 1.14
ES lysis ID8 20.03 1.74 1.1

Cloning of PCR product