Rui:Collaborations: Difference between revisions
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===Quick note=== | ===Quick note=== | ||
{| {{table}} border=1 | |||
| align="center" style="background:#f0f0f0;"|'''50 cells RNAseq''' | |||
| align="center" style="background:#f0f0f0;"|'''procedure''' | |||
| align="center" style="background:#f0f0f0;"|'''samples''' | |||
| align="center" style="background:#f0f0f0;"|'''start''' | |||
| align="center" style="background:#f0f0f0;"|'''end''' | |||
| align="center" style="background:#f0f0f0;"|'''note''' | |||
|- | |||
| ||50 cells RNAseq||RNA or lysate||Sperm vs ES cells||5.19.11||5.220.11 | |||
|- | |||
| ||||process, clone||5.21.11||5.24.11||5.25.11 seq result | |||
|- | |||
| ||||Nextera library||5.23.11||5.23.11||spread out (clone?) | |||
|- | |||
| ||||Solexa library||5.23.11||5.23.11||amp. Not good | |||
|- | |||
| ||||||5.26.11||5.26.11||again. Not good | |||
|- | |||
| ||||||6.1.11||6.2.11||different buffs | |||
|- | |||
| ||||||6.2.11||6.3.11||another 2ug repeat | |||
|- | |||
| ||||||6.4.11||||Kapa for NGS | |||
|} | |||
===2011=== | ===2011=== |
Revision as of 18:04, 17 June 2011
Dr. Yi Zhang lab
Samples
- Sperms: ~50cell/tube; 15 tubes
- ES cells: ~50cell/tube; 15 tubes
- E9.5 bag: ~641 cells in 1 tube; ~316 cells in 1 tube.
- E11.5 bag: ~21550 cells in 1 tube.
- E13.5 bag: ~30078 cells in 1 tube (male PGCs); ~57059 cells in 1 tube (female PGCs)
Quick note
50 cells RNAseq | procedure | samples | start | end | note |
50 cells RNAseq | RNA or lysate | Sperm vs ES cells | 5.19.11 | 5.220.11 | |
process, clone | 5.21.11 | 5.24.11 | 5.25.11 seq result | ||
Nextera library | 5.23.11 | 5.23.11 | spread out (clone?) | ||
Solexa library | 5.23.11 | 5.23.11 | amp. Not good | ||
5.26.11 | 5.26.11 | again. Not good | |||
6.1.11 | 6.2.11 | different buffs | |||
6.2.11 | 6.3.11 | another 2ug repeat | |||
6.4.11 | Kapa for NGS |
2011
<calendar> name=Rui:LabNotes/SingleCell format=%name/%year-%month-%day date=2011/10/1 view=oneyear </calendar>