AlanFung:LabNotes/CTCF/2011-6-17: Difference between revisions

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>Alan6017518
(Created page with '==Objective== *Perform Sanger Sequencing on library prepared by Methyl-Seq DNA Sample Prep Kit *Ensure the library is well constructed and that we are able to generate what Epice…')
 
>Alan6017518
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==PCR amplification and preparation for Sanger Sequencing==
==PCR amplification and preparation for Sanger Sequencing==
[[File:ZhangLab_2 2011-06-16 16hr 31min.jpg|400px]]
[[File:ZhangLab_2 2011-06-16 16hr 31min.jpg|400px]]
*Picked clone #1-6 for sanger sequencing
==Sequencing Results==
==Sequencing Results==

Revision as of 18:35, 17 June 2011

Objective

  • Perform Sanger Sequencing on library prepared by Methyl-Seq DNA Sample Prep Kit
  • Ensure the library is well constructed and that we are able to generate what Epicenter claimed to be the problem

Protocol

TA Cloning

Ligation of PCR vector

Transformation

PCR amplification and preparation for Sanger Sequencing

File:ZhangLab 2 2011-06-16 16hr 31min.jpg

  • Picked clone #1-6 for sanger sequencing

Sequencing Results