AlanFung:LabNotes/CTCF/2011-6-30: Difference between revisions
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#Nextera Library Construction (Low input protocol) | #Nextera Library Construction (Low input protocol) | ||
#PAGE Analysis | #PAGE Analysis | ||
==Protocol== | |||
==Proteinase K/Protease Digestion== |
Revision as of 18:52, 30 June 2011
N9-MDA Bisulfite Converted Sequencing Library Construction
Objective
- Figure out source of contamination from 6/28 experiment
- Repeat 6/28 experiment with only gDNA (UV EB buffer, use RT-PCR grade water as NTC)
- Sample List
- gDNA W 0.1ul proteinase K
- gDNA W proteinase k
- gDNA W/O proteinase k
- gDNA W protease
- NTC W proteinase K
- NTC W/O proteinase K
Overview
- Proteinase K Digestion
- Bisulfite Conversion
- Heat Denature
- MDA
- Ethanol Precipitation
- Nextera Library Construction (Low input protocol)
- PAGE Analysis