AlanFung:LabNotes/CTCF/2011-6-30: Difference between revisions

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#Nextera Library Construction (Low input protocol)
#Nextera Library Construction (Low input protocol)
#PAGE Analysis
#PAGE Analysis
==Protocol==
==Proteinase K/Protease Digestion==

Revision as of 18:52, 30 June 2011

N9-MDA Bisulfite Converted Sequencing Library Construction

Objective

  • Figure out source of contamination from 6/28 experiment
  • Repeat 6/28 experiment with only gDNA (UV EB buffer, use RT-PCR grade water as NTC)
  • Sample List
  1. gDNA W 0.1ul proteinase K
  2. gDNA W proteinase k
  3. gDNA W/O proteinase k
  4. gDNA W protease
  5. NTC W proteinase K
  6. NTC W/O proteinase K

Overview

  1. Proteinase K Digestion
  2. Bisulfite Conversion
  3. Heat Denature
  4. MDA
  5. Ethanol Precipitation
  6. Nextera Library Construction (Low input protocol)
  7. PAGE Analysis

Protocol

Proteinase K/Protease Digestion