AlanFung:LabNotes/CTCF/2011-6-30: Difference between revisions
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*Repeat 6/28 experiment with only gDNA (UV EB buffer, use RT-PCR grade water as NTC) | *Repeat 6/28 experiment with only gDNA (UV EB buffer, use RT-PCR grade water as NTC) | ||
*Sample List | *Sample List | ||
#A-gDNA W 0.1ul proteinase K | #A-600pg gDNA W 0.1ul proteinase K | ||
#B-gDNA W proteinase k | #B-600pg gDNA W proteinase k | ||
#C-gDNA W/O proteinase k | #C-600pg gDNA W/O proteinase k | ||
#D-gDNA W protease | #D-600pg gDNA W protease | ||
#E-NTC W proteinase K | #E-NTC W proteinase K | ||
#F-NTC W/O proteinase K | #F-NTC W/O proteinase K |
Revision as of 18:56, 30 June 2011
N9-MDA Bisulfite Converted Sequencing Library Construction
Objective
- Figure out source of contamination from 6/28 experiment
- Repeat 6/28 experiment with only gDNA (UV EB buffer, use RT-PCR grade water as NTC)
- Sample List
- A-600pg gDNA W 0.1ul proteinase K
- B-600pg gDNA W proteinase k
- C-600pg gDNA W/O proteinase k
- D-600pg gDNA W protease
- E-NTC W proteinase K
- F-NTC W/O proteinase K
Overview
- Proteinase K/Protease Digestion
- Bisulfite Conversion
- Heat Denature
- MDA
- Ethanol Precipitation
- Nextera Library Construction (Low input protocol)
- PAGE Analysis