Rui:LabNotes/SingleCell/2011-7-1: Difference between revisions
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EtOH precipitation: 5ul NaOAc, 125ul EtOH, -80C 30min, spin 30min, huge pellet, resuspend in 30ul | EtOH precipitation: 5ul NaOAc, 125ul EtOH, -80C 30min, spin 30min, huge pellet, resuspend in 30ul | ||
Qbuit: L2: 110ng/ul; L3: 10ng/ul; PC out of range (too high) | |||
[[File:7.6.11_quant.jpg]] | [[File:7.6.11_quant.jpg]] | ||
[[File:7.6.11_over.jpg]] | [[File:7.6.11_over.jpg]] |
Revision as of 18:19, 7 July 2011
Purification of HRCA results
MinElute
NC, PC, 60pg gDNA, 1pg lysate 1, elute in 10ul, 1ul for TBE PAGE
Qbuit: ~1ng/ul
EtOH precipitation (ExoI treatment)
PC, 1pg lysate 2 (treated with ExoI for 30min)
PC, 1pg lysate 3 (no treatment)
EtOH precipitation: 5ul NaOAc, 125ul EtOH, -80C 30min, spin 30min, huge pellet, resuspend in 30ul
Qbuit: L2: 110ng/ul; L3: 10ng/ul; PC out of range (too high)