Rui:LabNotes/Collaborations/2011-7-8: Difference between revisions

From ZhangLabWiki
Jump to navigation Jump to search
>RuiLiu
m (Created page with '==Quantification by gel== ==Covaris shearing DNAs== # Add 66ul purification buffer to ~8ul DNA samples # Transfer 75ul mixture to Covaris tube # Covaris location: Cancer center,…')
 
>RuiLiu
 
Line 16: Line 16:
  |}
  |}


# Quick spin and transfer to PCR tubes
# Quick spin and transfer to PCR tubes if continue with library progress; if stop, store DNA in non-stick tube (1.5ml or 0.5ml)

Latest revision as of 16:24, 13 July 2011

Quantification by gel[edit]

Covaris shearing DNAs[edit]

  1. Add 66ul purification buffer to ~8ul DNA samples
  2. Transfer 75ul mixture to Covaris tube
  3. Covaris location: Cancer center, 3rd, Rm.3352; Open Kun Zhang lab folder, use 200bp-5min.pro file; put sample in; start
Duty% Intensity Burst cycle Time (min) Mode
10 5 200 5min frequency sweeping
  1. Quick spin and transfer to PCR tubes if continue with library progress; if stop, store DNA in non-stick tube (1.5ml or 0.5ml)