Rui:LabNotes/Collaborations/2011-7-11: Difference between revisions

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>RuiLiu
m (Created page with '==Prepare RNAseq library for sequencing== * Continue with lab note on 7/8/11 * Samples: PC1, PC2, GFP+, GFP-, E9.5, E11.5, E13.5m, E13.5f * 75ul sheared DNA store at 0.5ml non-st…')
 
>RuiLiu
Line 11: Line 11:
  Enzyme mix            5ul
  Enzyme mix            5ul
  H2O                  10ul
  H2O                  10ul
''' Total                100ul'''
''' Total                100ul'''
  20C for 30min
  20C for 30min



Revision as of 16:52, 11 July 2011

Prepare RNAseq library for sequencing

  • Continue with lab note on 7/8/11
  • Samples: PC1, PC2, GFP+, GFP-, E9.5, E11.5, E13.5m, E13.5f
  • 75ul sheared DNA store at 0.5ml non-stick tube over the weekend
  • Use NEB master mix kit for Library prep

End repair

Reaction 
sheared DNA           75ul
Master mix buffer     10ul
Enzyme mix             5ul
H2O                   10ul
 Total                100ul
20C for 30min

A tailing

=Adapter ligation

PCR amplification

Size selection