Rui:LabNotes/Collaborations/2011-7-11: Difference between revisions
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>RuiLiu m (Created page with '==Prepare RNAseq library for sequencing== * Continue with lab note on 7/8/11 * Samples: PC1, PC2, GFP+, GFP-, E9.5, E11.5, E13.5m, E13.5f * 75ul sheared DNA store at 0.5ml non-st…') |
>RuiLiu m (→End repair) |
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Line 11: | Line 11: | ||
Enzyme mix 5ul | Enzyme mix 5ul | ||
H2O 10ul | H2O 10ul | ||
''' Total 100ul''' | ''' Total 100ul''' | ||
20C for 30min | 20C for 30min | ||
Revision as of 16:52, 11 July 2011
Prepare RNAseq library for sequencing
- Continue with lab note on 7/8/11
- Samples: PC1, PC2, GFP+, GFP-, E9.5, E11.5, E13.5m, E13.5f
- 75ul sheared DNA store at 0.5ml non-stick tube over the weekend
- Use NEB master mix kit for Library prep
End repair
Reaction sheared DNA 75ul Master mix buffer 10ul Enzyme mix 5ul H2O 10ul Total 100ul 20C for 30min