Rui:LabNotes/Collaborations/2011-7-12: Difference between revisions

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>RuiLiu
(Created page with '==Prepare RNAseq library for sequencing== ===Size selection=== * 0.7X beads (35ul) to 50ul PCR, elute with 15ul * Transfer 12ul to new tube for sequencing, 1ul left for gel, 1ul…')
 
>RuiLiu
Line 9: Line 9:


Qubit concentration
Qubit concentration
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Sample'''
| align="center" style="background:#f0f0f0;"|'''ng/ul'''
| align="center" style="background:#f0f0f0;"|'''total ng'''
|-
| PC1||13.2||158.4
|-
| PC2||11.8||141.6
|-
| GFP+||7.67||92.04
|-
| GFP-||11||132
|-
| E9.5||6.99||83.88
|-
| E11.5||11.4||136.8
|-
| E13.5m||12.4||148.8
|-
| E13.5f||10.5||126
|}

Revision as of 18:33, 12 July 2011

Prepare RNAseq library for sequencing

Size selection

  • 0.7X beads (35ul) to 50ul PCR, elute with 15ul
  • Transfer 12ul to new tube for sequencing, 1ul left for gel, 1ul left for Qubit

TBE PAGE File:7.12.11 final check.jpg

Qubit concentration

Sample ng/ul total ng
PC1 13.2 158.4
PC2 11.8 141.6
GFP+ 7.67 92.04
GFP- 11 132
E9.5 6.99 83.88
E11.5 11.4 136.8
E13.5m 12.4 148.8
E13.5f 10.5 126