Rui:LabNotes/Collaborations/2011-7-12: Difference between revisions
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>RuiLiu (Created page with '==Prepare RNAseq library for sequencing== ===Size selection=== * 0.7X beads (35ul) to 50ul PCR, elute with 15ul * Transfer 12ul to new tube for sequencing, 1ul left for gel, 1ul…') |
>RuiLiu |
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Qubit concentration | Qubit concentration | ||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Sample''' | |||
| align="center" style="background:#f0f0f0;"|'''ng/ul''' | |||
| align="center" style="background:#f0f0f0;"|'''total ng''' | |||
|- | |||
| PC1||13.2||158.4 | |||
|- | |||
| PC2||11.8||141.6 | |||
|- | |||
| GFP+||7.67||92.04 | |||
|- | |||
| GFP-||11||132 | |||
|- | |||
| E9.5||6.99||83.88 | |||
|- | |||
| E11.5||11.4||136.8 | |||
|- | |||
| E13.5m||12.4||148.8 | |||
|- | |||
| E13.5f||10.5||126 | |||
|} |
Revision as of 18:33, 12 July 2011
Prepare RNAseq library for sequencing
Size selection
- 0.7X beads (35ul) to 50ul PCR, elute with 15ul
- Transfer 12ul to new tube for sequencing, 1ul left for gel, 1ul left for Qubit
TBE PAGE File:7.12.11 final check.jpg
Qubit concentration
Sample | ng/ul | total ng |
PC1 | 13.2 | 158.4 |
PC2 | 11.8 | 141.6 |
GFP+ | 7.67 | 92.04 |
GFP- | 11 | 132 |
E9.5 | 6.99 | 83.88 |
E11.5 | 11.4 | 136.8 |
E13.5m | 12.4 | 148.8 |
E13.5f | 10.5 | 126 |