Rui:LabNotes/Collaborations/2011-7-12: Difference between revisions
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>RuiLiu |
>RuiLiu m (→Size selection) |
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===Size selection=== | ===Size selection=== | ||
====Beads purification==== | |||
* 0.7X beads (35ul) to 50ul PCR, elute with 15ul | * 0.7X beads (35ul) to 50ul PCR, elute with 15ul | ||
* Transfer 12ul to new tube for sequencing, 1ul left for gel, 1ul left for Qubit | * Transfer 12ul to new tube for sequencing, 1ul left for gel, 1ul left for Qubit | ||
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| E13.5f||10.5||126 | | E13.5f||10.5||126 | ||
|} | |} | ||
====Gel purification==== |
Revision as of 18:36, 12 July 2011
Prepare RNAseq library for sequencing
Size selection
Beads purification
- 0.7X beads (35ul) to 50ul PCR, elute with 15ul
- Transfer 12ul to new tube for sequencing, 1ul left for gel, 1ul left for Qubit
TBE PAGE File:7.12.11 final check.jpg
Qubit concentration
Sample | ng/ul | total ng |
PC1 | 13.2 | 158.4 |
PC2 | 11.8 | 141.6 |
GFP+ | 7.67 | 92.04 |
GFP- | 11 | 132 |
E9.5 | 6.99 | 83.88 |
E11.5 | 11.4 | 136.8 |
E13.5m | 12.4 | 148.8 |
E13.5f | 10.5 | 126 |