Rui:LabNotes/Collaborations/2011-7-12: Difference between revisions

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===Size selection===
===Size selection===
====Beads purification====
* 0.7X beads (35ul) to 50ul PCR, elute with 15ul
* 0.7X beads (35ul) to 50ul PCR, elute with 15ul
* Transfer 12ul to new tube for sequencing, 1ul left for gel, 1ul left for Qubit
* Transfer 12ul to new tube for sequencing, 1ul left for gel, 1ul left for Qubit
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| E13.5f||10.5||126
| E13.5f||10.5||126
|}
|}
====Gel purification====

Revision as of 18:36, 12 July 2011

Prepare RNAseq library for sequencing

Size selection

Beads purification

  • 0.7X beads (35ul) to 50ul PCR, elute with 15ul
  • Transfer 12ul to new tube for sequencing, 1ul left for gel, 1ul left for Qubit

TBE PAGE File:7.12.11 final check.jpg

Qubit concentration

Sample ng/ul total ng
PC1 13.2 158.4
PC2 11.8 141.6
GFP+ 7.67 92.04
GFP- 11 132
E9.5 6.99 83.88
E11.5 11.4 136.8
E13.5m 12.4 148.8
E13.5f 10.5 126

Gel purification