Rui:LabNotes/Collaborations/2011-7-12: Difference between revisions
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>RuiLiu m (→Size selection) |
>RuiLiu |
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====Gel purification==== | ====Gel purification==== | ||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Samples''' | |||
| align="center" style="background:#f0f0f0;"|'''gel intensity''' | |||
| align="center" style="background:#f0f0f0;"|'''relative intensity''' | |||
| align="center" style="background:#f0f0f0;"|'''[conc.]''' | |||
| align="center" style="background:#f0f0f0;"|'''V for 100ng''' | |||
| align="center" style="background:#f0f0f0;"|'''Qubit [conc.]''' | |||
|- | |||
| GFP+||221464.69||120725.2||40.77||2.55||7.67 | |||
|- | |||
| GFP-||257057.98||152022.4||51.97||2||11 | |||
|- | |||
| E9.5||207727.09||102391.96||34.21||3.04||6.99 | |||
|- | |||
| E11.5||233881.54||128290.35||43.48||2.39||11.4 | |||
|- | |||
| E13.5m||245476.91||132162.7||44.86||2.32||12.4 | |||
|- | |||
| E13.5f||219923.48||120021.7||40.52||2.57||10.5 | |||
|- | |||
| | |||
|} |
Revision as of 00:14, 13 July 2011
Prepare RNAseq library for sequencing
Size selection
Beads purification
- 0.7X beads (35ul) to 50ul PCR, elute with 15ul
- Transfer 12ul to new tube for sequencing, 1ul left for gel, 1ul left for Qubit
TBE PAGE File:7.12.11 final check.jpg
Qubit concentration
Sample | ng/ul | total ng |
PC1 | 13.2 | 158.4 |
PC2 | 11.8 | 141.6 |
GFP+ | 7.67 | 92.04 |
GFP- | 11 | 132 |
E9.5 | 6.99 | 83.88 |
E11.5 | 11.4 | 136.8 |
E13.5m | 12.4 | 148.8 |
E13.5f | 10.5 | 126 |
Gel purification
Samples | gel intensity | relative intensity | [conc.] | V for 100ng | Qubit [conc.] |
GFP+ | 221464.69 | 120725.2 | 40.77 | 2.55 | 7.67 |
GFP- | 257057.98 | 152022.4 | 51.97 | 2 | 11 |
E9.5 | 207727.09 | 102391.96 | 34.21 | 3.04 | 6.99 |
E11.5 | 233881.54 | 128290.35 | 43.48 | 2.39 | 11.4 |
E13.5m | 245476.91 | 132162.7 | 44.86 | 2.32 | 12.4 |
E13.5f | 219923.48 | 120021.7 | 40.52 | 2.57 | 10.5 |