Rui:SingelCell RNAseq: Difference between revisions

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>Jdeng
>Jdeng
Line 21: Line 21:
*[[Rui:IVT note|IVT note]]
*[[Rui:IVT note|IVT note]]
*[[Rui:HRCA note|HRCA note]]
*[[Rui:HRCA note|HRCA note]]
===IVT note===
{| {{table}} border=1
| align="center" style="background:#f0f0f0;"|'''IVT_based'''
| align="center" style="background:#f0f0f0;"|'''description'''
| align="center" style="background:#f0f0f0;"|'''start'''
| align="center" style="background:#f0f0f0;"|'''result'''
|-
| 1st try||3 sets||2.8.11||2.9.11
|-
| Following||purification?||2.10.11||
|-
| ||primer check||2.15.11||
|-
| ||clone||2.17.11||2.24.11
|-
| ||clone troubleshoot||2.28.11||
|-
| 2nd round IVT||||3.2.11||3.3.11
|-
| ||trouble shooting||3.3.11||3.4.11
|-
| Atailed_ds cDNA IVT||||3.2.11||3.3.11
|-
| 2nd try||repeat kit||3.8.11||3.9.11
|-
| ||||3.10.11||3.11.11
|-
| Atailed_ds cDNA IVT||||4.5.11||4.6.11
|}


===HRCA note===
===HRCA note===

Revision as of 19:15, 14 November 2012

Background

  • Goal: the smallest functional unit; the exact quantity; full-length RNAs; sense vs anti-sense; all classes; at single-base resolution
  • Review papers:
  • Key papers:
  1. Single cell and cell lysis
  2. 1st cDNA synthesis and free primer removal
  3. 2nd cDNA synthesis: A-tailing vs random
  4. Amplification: PCR or IVT
  5. QC and clone

Protocols

QuickNotes

HRCA note

HRCA description start result
1st try P1 2.3.11 2.4.11

2012

<calendar> name=Rui:LabNotes/SingleCell format=%name/%year-%month-%day date=2012/10/1 view=oneyear </calendar>


2011

<calendar> name=Rui:LabNotes/SingleCell format=%name/%year-%month-%day date=2011/10/1 view=oneyear </calendar>