AlanFung:LabNotes/Methylome/2012-1-17: Difference between revisions

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==GM12878 Library==
==GM12878 Library==
==Sample Preparation==
==Sample Preparation==
*Get GM12878 samples from Noi
*Covaris shearing of 5ug gDNA to ~400bp
*Covaris shearing of 5ug gDNA to ~400bp
*Contacted Yang at Kelly Frazer's lab to help me with it
*Contacted Yang at Kelly Frazer's lab to help me with it
==End repair & A-tailing==
==End repair & A-tailing==
==ligation to methylated Y adaptor==
==ligation to methylated Y adaptor==

Revision as of 22:32, 17 January 2012

GM12878 Library

Sample Preparation

  • Get GM12878 samples from Noi
  • Covaris shearing of 5ug gDNA to ~400bp
  • Contacted Yang at Kelly Frazer's lab to help me with it

End repair & A-tailing

ligation to methylated Y adaptor

Bisulfite Conversion

PCR

Size selection