AlanFung:LabNotes/Methylome/2012-1-17: Difference between revisions
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==GM12878 Library== | ==GM12878 Library== | ||
==Sample Preparation== | ==Sample Preparation== | ||
*Get GM12878 samples from Noi | |||
*Covaris shearing of 5ug gDNA to ~400bp | *Covaris shearing of 5ug gDNA to ~400bp | ||
*Contacted Yang at Kelly Frazer's lab to help me with it | *Contacted Yang at Kelly Frazer's lab to help me with it | ||
==End repair & A-tailing== | ==End repair & A-tailing== | ||
==ligation to methylated Y adaptor== | ==ligation to methylated Y adaptor== |
Revision as of 22:32, 17 January 2012
GM12878 Library
Sample Preparation
- Get GM12878 samples from Noi
- Covaris shearing of 5ug gDNA to ~400bp
- Contacted Yang at Kelly Frazer's lab to help me with it