AlanFung:LabNotes/Methylome/2012-1-17: Difference between revisions
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==End repair & A-tailing== | ==End repair & A-tailing== | ||
==ligation to methylated Y adaptor== | ==ligation to methylated Y adaptor== | ||
==Bisulfite Conversion== | ==Bisulfite Conversion== | ||
==PCR== | ==PCR== | ||
==Size selection== | ==Size selection== |
Revision as of 22:49, 17 January 2012
GM12878 Library
Sample Preparation
- Get GM12878 samples from Noi
- Measure concentration with qubit
- Take 5ug of samples and prepare for shearing (Make total volume to 100ul with ddh2o)
- Covaris shearing of 5ug gDNA to ~400bp
- Contacted Yang at Kelly Frazer's lab to help me with it