AlanFung:LabNotes/Methylome/2012-1-17: Difference between revisions

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==End repair & A-tailing==
==End repair & A-tailing==
==ligation to methylated Y adaptor==
==ligation to methylated Y adaptor==
==Bisulfite Conversion==
==Bisulfite Conversion==
==PCR==
==PCR==
==Size selection==
==Size selection==

Revision as of 22:49, 17 January 2012

GM12878 Library

Sample Preparation

  • Get GM12878 samples from Noi
  • Measure concentration with qubit
  • Take 5ug of samples and prepare for shearing (Make total volume to 100ul with ddh2o)
  • Covaris shearing of 5ug gDNA to ~400bp
  • Contacted Yang at Kelly Frazer's lab to help me with it

End repair & A-tailing

ligation to methylated Y adaptor

Bisulfite Conversion

PCR

Size selection