Brandon:Protocols/primers prep: Difference between revisions

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>Bsos
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==Primers preparation==
==Primers preparation==


*for each nmol (nm) of primer add 10 uL nuclease free H2O. ie 35.5 um, add 355 uL nuclease free water.  now is at stock concentration of 100 uM.
*for each nmol (nm) of primer add 10 uL nuclease free H2O. ie 35.5 nm, add 355 uL nuclease free water.  now is at stock concentration of 100 umol.
*next dilute to usable [] of 10 uM with 10X dilution.
*next dilute to usable [] of 10 uM with 10X dilution.
*combine F/R primers in one tube for usable primer.
*combine F/R primers in one tube for usable primer.

Revision as of 03:05, 29 June 2013

Primers preparation

  • for each nmol (nm) of primer add 10 uL nuclease free H2O. ie 35.5 nm, add 355 uL nuclease free water. now is at stock concentration of 100 umol.
  • next dilute to usable [] of 10 uM with 10X dilution.
  • combine F/R primers in one tube for usable primer.