Brandon:Protocols/primers prep: Difference between revisions

From ZhangLabWiki
Jump to navigation Jump to search
>Bsos
>Bsos
 
Line 1: Line 1:
==Primers preparation==
==Primers preparation==


*for each nmol (nm) of primer add 10 uL nuclease free H2O. ie 35.5 nm, add 355 uL nuclease free water.  now is at stock concentration of 100 umol.
*for each nmol (nm) of primer add 10 uL nuclease free H2O. ie 35.5 nm, add 355 uL nuclease free water.  now is at stock concentration of 100 uM (molar).
*next dilute to usable [] of 10 uM with 10X dilution.
*next dilute to usable [] of 10 uM with 10X dilution.
*combine F/R primers in one tube for usable primer.
*combine F/R primers in one tube for usable primer.

Latest revision as of 03:07, 29 June 2013

Primers preparation[edit]

  • for each nmol (nm) of primer add 10 uL nuclease free H2O. ie 35.5 nm, add 355 uL nuclease free water. now is at stock concentration of 100 uM (molar).
  • next dilute to usable [] of 10 uM with 10X dilution.
  • combine F/R primers in one tube for usable primer.