Brandon:Protocols/primers prep: Difference between revisions
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==Primers preparation== | ==Primers preparation== | ||
*for each nmol (nm) of primer add 10 uL nuclease free H2O. ie 35.5 nm, add 355 uL nuclease free water. now is at stock concentration of 100 | *for each nmol (nm) of primer add 10 uL nuclease free H2O. ie 35.5 nm, add 355 uL nuclease free water. now is at stock concentration of 100 uM (molar). | ||
*next dilute to usable [] of 10 uM with 10X dilution. | *next dilute to usable [] of 10 uM with 10X dilution. | ||
*combine F/R primers in one tube for usable primer. | *combine F/R primers in one tube for usable primer. |
Latest revision as of 03:07, 29 June 2013
Primers preparation[edit]
- for each nmol (nm) of primer add 10 uL nuclease free H2O. ie 35.5 nm, add 355 uL nuclease free water. now is at stock concentration of 100 uM (molar).
- next dilute to usable [] of 10 uM with 10X dilution.
- combine F/R primers in one tube for usable primer.