Rui:LabNotes/DNA Collaborations/2012-5-10: Difference between revisions

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>RuiLiu
(Created page with "==Continue with D2 and D3== # EtOH precipitation o/n # Spin down with 3700 rpm, really difficult handling with pellet - need to be careful. Resupend in 5ul, 1ul for AluPCR - ...")
 
>RuiLiu
 
Line 10: Line 10:


===Results===
===Results===
[[File:5.10.12_D2.2.jpg|400px]][[File:5.10.12_D-p0.jpg|400px]]
Test on D2.2 [[File:5.10.12_D2.2.jpg|400px]] D2 [[File:5.10.12_D2.jpg|400px]] D3 [[File:5.10.12_D3.jpg|400px]]


[[File:5.10.12_D2.2-gel.jpg|400px]][[File:5.10.12_D2.1-gel.jpg|400px]]
[[File:5.10.12_D2.2-gel.jpg|400px]][[File:5.10.12_D2.1-gel.jpg|400px]]

Latest revision as of 00:02, 12 May 2012

Continue with D2 and D3[edit]

  1. EtOH precipitation o/n
  2. Spin down with 3700 rpm, really difficult handling with pellet - need to be careful. Resupend in 5ul, 1ul for AluPCR - failed to amplify (due to primer pre-mix?? why??)
  3. 2ul DNA for Tn tagmentation in 5ul total
  4. Protease treatment for 30min
  5. PCR with adaptor 1 and barcoded adaptor 2
  6. Beads purification for 48 samples
  7. Gel quantification of 48 samples, 24 for one library
  8. Mixed library for size selection

Results[edit]

Test on D2.2 File:5.10.12 D2.2.jpg D2 File:5.10.12 D2.jpg D3 File:5.10.12 D3.jpg

File:5.10.12 D2.2-gel.jpgFile:5.10.12 D2.1-gel.jpg