Rui:LabNotes/DNA Collaborations/2012-5-10: Difference between revisions
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>RuiLiu (Created page with "==Continue with D2 and D3== # EtOH precipitation o/n # Spin down with 3700 rpm, really difficult handling with pellet - need to be careful. Resupend in 5ul, 1ul for AluPCR - ...") |
>RuiLiu m (→Results) |
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===Results=== | ===Results=== | ||
[[File:5.10.12_D2.2.jpg|400px]][[File:5.10. | Test on D2.2 [[File:5.10.12_D2.2.jpg|400px]] D2 [[File:5.10.12_D2.jpg|400px]] D3 [[File:5.10.12_D3.jpg|400px]] | ||
[[File:5.10.12_D2.2-gel.jpg|400px]][[File:5.10.12_D2.1-gel.jpg|400px]] | [[File:5.10.12_D2.2-gel.jpg|400px]][[File:5.10.12_D2.1-gel.jpg|400px]] |
Latest revision as of 00:02, 12 May 2012
Continue with D2 and D3[edit]
- EtOH precipitation o/n
- Spin down with 3700 rpm, really difficult handling with pellet - need to be careful. Resupend in 5ul, 1ul for AluPCR - failed to amplify (due to primer pre-mix?? why??)
- 2ul DNA for Tn tagmentation in 5ul total
- Protease treatment for 30min
- PCR with adaptor 1 and barcoded adaptor 2
- Beads purification for 48 samples
- Gel quantification of 48 samples, 24 for one library
- Mixed library for size selection
Results[edit]
Test on D2.2 File:5.10.12 D2.2.jpg D2 File:5.10.12 D2.jpg D3 File:5.10.12 D3.jpg