AlanFung:LabNotes/Capturing/2012-5-15: Difference between revisions
Jump to navigation
Jump to search
>Alan6017518 |
>Alan6017518 |
||
Line 112: | Line 112: | ||
*From the gel quantification we have 29.47ng/ul x 80ul =2.36ug of probes | *From the gel quantification we have 29.47ng/ul x 80ul =2.36ug of probes | ||
*Probe:target ratio: 200:1 | *Probe:target ratio: 200:1 | ||
H7 | |||
H14 |
Revision as of 23:17, 22 May 2012
Sample List
ChIPK36me3 | GM12878 |
ChIPK36me3 | P10 |
ChIPK36me3 | DF2 |
ChIPK36me3 | H0 |
ChIPK36me3 | H7 |
ChIPK36me3 | H14 |
ChIPK36me3 | H16 |
ChIPK27me3 | GM12878 |
ChIPK27me3 | P10 |
ChIPK27me3 | DF2 |
ChIPK27me3 | H0 |
ChIPK27me3 | H7 |
ChIPK27me3 | H14 |
ChIPK27me3 | H16 |
WCE | GM12878 |
WCE | P10 |
WCE | DF2 |
WCE | H0 |
WCE | H7 |
WCE | H14 |
WCE | H16 |
GM | cDNA |
P10E3 | cDNA |
DF2 | cDNA |
H0 | cDNA |
H7 | cDNA |
H14 | cDNA |
H16 | cDNA |
GM | gDNA |
P10E3 | gDNA |
DF2 | gDNA |
H0 | gDNA |
H7 | gDNA |
H14 | gDNA |
H16 | gDNA |
GmgDNA:H0gDNA::1:1 | 38 |
Sample
- H7 and H14 gDNA has the most amount and I would like to use these two samples for a first test
Sample ID | User ID | Date | Time | ng/ul | A260 | A280 | 260/280 | 260/230 | Constant | Cursor Pos. | Cursor abs. | 340 raw | Vol | Total | to get 1000ng | Leftover for me |
H7 gDNA | Default | 4/5/2012 | 3:57 PM | 184.33 | 3.687 | 1.787 | 2.06 | 2.23 | 50 | 230 | 1.652 | 0.001 | 100 | 18433 | 5.43 | 94.6 |
H14 gDNA | Default | 4/5/2012 | 3:58 PM | 167.18 | 3.344 | 1.669 | 2 | 2.17 | 50 | 230 | 1.541 | 0.016 | 100 | 16718 | 5.98 | 94.0 |
Probes Needed
- Amount of probes
- From the gel quantification we have 29.47ng/ul x 80ul =2.36ug of probes
- Probe:target ratio: 200:1
H7 H14