Brandon:Protocols/taq amplification2: Difference between revisions
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>Bsos (Created page with "==taq amplification== used taq2x for amplification, X35 cycles 12.5 uL taq2X 2 uL primers, 1 uL F, 1 uL R 5.5 uL H2O 5 uL DNA template taq2x cycles: 95C 5min, ...") |
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5.5 uL H2O | 5.5 uL H2O | ||
5 uL DNA template | 5 uL DNA template | ||
taq2x cycles: | taq2x cycles: | ||
95C 5min, (95C for 30s, 60C for 30s, 72C for 1 min) X35, 72C for 5 min, 4C forever | 95C 5min, (95C for 30s, 60C for 30s, 72C for 1 min) X35, 72C for 5 min, 4C forever |
Revision as of 21:25, 17 May 2012
taq amplification
used taq2x for amplification, X35 cycles
12.5 uL taq2X 2 uL primers, 1 uL F, 1 uL R 5.5 uL H2O 5 uL DNA template taq2x cycles: 95C 5min, (95C for 30s, 60C for 30s, 72C for 1 min) X35, 72C for 5 min, 4C forever