Brandon:Protocols/taq amplification2: Difference between revisions

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>Bsos
(Created page with "==taq amplification== used taq2x for amplification, X35 cycles 12.5 uL taq2X 2 uL primers, 1 uL F, 1 uL R 5.5 uL H2O 5 uL DNA template taq2x cycles: 95C 5min, ...")
 
>Bsos
Line 7: Line 7:
  5.5  uL H2O
  5.5  uL H2O
  5    uL DNA template
  5    uL DNA template
 
  taq2x cycles:
  taq2x cycles:
  95C 5min, (95C for 30s, 60C for 30s, 72C for 1 min) X35, 72C for 5 min, 4C forever
  95C 5min, (95C for 30s, 60C for 30s, 72C for 1 min) X35, 72C for 5 min, 4C forever

Revision as of 21:25, 17 May 2012

taq amplification

used taq2x for amplification, X35 cycles

12.5 uL taq2X
2    uL primers, 1 uL F, 1 uL R
5.5  uL H2O
5    uL DNA template

taq2x cycles:
95C 5min, (95C for 30s, 60C for 30s, 72C for 1 min) X35, 72C for 5 min, 4C forever