Tina:MESC rainbow line: Difference between revisions

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>TinaLo
(Created page with "==mESC rainbow line expansion== *'''5/2''' **Received one vial of cells from Inna, and stored it in the N2(l) tank located at CERC. *'''5/3 discussed with Dr. Blue Lake in D...")
 
>TinaLo
Line 22: Line 22:


*'''5/21 Passaging (Blue and Tina)'''
*'''5/21 Passaging (Blue and Tina)'''
**1:3 wells look very healthy; 1:6 wells look good, and 1:12 well can wait for one more day
**1:3 wells look very healthy; 1:6 wells look good, and 1:12 well can wait for one more day.
**Passage one well of 1:3 into 1:3 (1 well), 1:6 (1 well) and 1:12 (1 well)
**Passage one well of 1:3 into 1:3 (1 well), 1:6 (1 well) and 1:12 (1 well)
**Passage one well of 1:6 into 1:3 (1 well), 1:6 (1 well) and 1:12 (1 well)
**Passage one well of 1:6 into 1:3 (1 well), 1:6 (1 well) and 1:12 (1 well)
**Frozen down 8 vials of P5 InsACGGsAlov3 (from one 1:3 well and one 1:6 well)
**Frozen down 8 vials of P5 InsACGGsAlov3 (from one 1:3 well and one 1:6 well)
*'''5/22'''
**P6 cells look nice. Cells were passaged well yesterday.
**P5 1:12 well shows a few differentiated colonies, and therefore is discarded to maintain good cell quality.

Revision as of 17:32, 22 May 2012

mESC rainbow line expansion

  • 5/2
    • Received one vial of cells from Inna, and stored it in the N2(l) tank located at CERC.
  • 5/3 discussed with Dr. Blue Lake in Dr. Yang Xu’s lab
  • 5/7 preparing MEF with Blue
  • 5/8 thawing
    • Thaw cells in one 6-well plate
  • 5/11
    • some colonies appear to show
  • 5/14 Passaging
    • The culture looks ok, but there is some differentiation… so Blue passaged at several different dilutions (the lower ones to try and get rid of the differentiated cells).
    • Passage one 6 well into one 1:3, one 1:4, one 1:5, and one 1:6 well.
  • 5/18 Passaging
    • Passage the 1:3 well into two 1:3 wells, two 1:6 wells, and one 1:12 well.
    • Frozen down 3 vials of P4 InsACGGsAlov3 (from one 1:4 well, one 1:5 well, and one 1:6 well).
  • 5/21 Passaging (Blue and Tina)
    • 1:3 wells look very healthy; 1:6 wells look good, and 1:12 well can wait for one more day.
    • Passage one well of 1:3 into 1:3 (1 well), 1:6 (1 well) and 1:12 (1 well)
    • Passage one well of 1:6 into 1:3 (1 well), 1:6 (1 well) and 1:12 (1 well)
    • Frozen down 8 vials of P5 InsACGGsAlov3 (from one 1:3 well and one 1:6 well)
  • 5/22
    • P6 cells look nice. Cells were passaged well yesterday.
    • P5 1:12 well shows a few differentiated colonies, and therefore is discarded to maintain good cell quality.