Tina:LabNotes/2012-6-1: Difference between revisions
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>TinaLo (Created page with "*Collect MEF conditioned medium (2*10-2=18 mL), store at -20. Add new mESC medium to MEF cells (including the one in T75 flask thawed yesterday). *Passage P8 InsACGGsAlov3 cel...") |
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*Collect MEF conditioned medium (2*10-2=18 mL), store at -20. Add new mESC medium to MEF cells (including the one in T75 flask thawed yesterday). | *Collect MEF conditioned medium (2*10-2=18 mL), store at -20. Add new mESC medium to MEF cells (including the one in T75 flask thawed yesterday). | ||
*Passage P8 InsACGGsAlov3 | *Passage one well of 1:12 P8 InsACGGsAlov3 mESCs into 1:6 (1 well) and 1:3 (1 well; on 0.1% gelatin coating plate without MEF feeder cells; add [[MEF conditioned media]]) | ||
*Transfer 3 vials of P8 InsACGGsAlov3 from -80 to N2(l) tank. | *Transfer 3 vials of P8 InsACGGsAlov3 from -80 to N2(l) tank. | ||
*Transfer all InsACGGsAlov3 c3lls into a new box (named "ZhangLab Box 3). Update cell storage information. | *Transfer all InsACGGsAlov3 c3lls into a new box (named "ZhangLab Box 3). Update cell storage information. | ||
*Transfer LIF from -80 to -20. | *Transfer LIF from -80 to -20. |
Latest revision as of 22:46, 4 June 2012
- Collect MEF conditioned medium (2*10-2=18 mL), store at -20. Add new mESC medium to MEF cells (including the one in T75 flask thawed yesterday).
- Passage one well of 1:12 P8 InsACGGsAlov3 mESCs into 1:6 (1 well) and 1:3 (1 well; on 0.1% gelatin coating plate without MEF feeder cells; add MEF conditioned media)
- Transfer 3 vials of P8 InsACGGsAlov3 from -80 to N2(l) tank.
- Transfer all InsACGGsAlov3 c3lls into a new box (named "ZhangLab Box 3). Update cell storage information.
- Transfer LIF from -80 to -20.