Tina:LabNotes/2012-6-1: Difference between revisions

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>TinaLo
(Created page with "*Collect MEF conditioned medium (2*10-2=18 mL), store at -20. Add new mESC medium to MEF cells (including the one in T75 flask thawed yesterday). *Passage P8 InsACGGsAlov3 cel...")
 
>TinaLo
mNo edit summary
 
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*Collect MEF conditioned medium (2*10-2=18 mL), store at -20. Add new mESC medium to MEF cells (including the one in T75 flask thawed yesterday).
*Collect MEF conditioned medium (2*10-2=18 mL), store at -20. Add new mESC medium to MEF cells (including the one in T75 flask thawed yesterday).
*Passage P8 InsACGGsAlov3 cells into P9 (one well on MEF; one well feeder free)
*Passage one well of 1:12 P8 InsACGGsAlov3 mESCs into 1:6 (1 well) and 1:3 (1 well; on 0.1% gelatin coating plate without MEF feeder cells; add [[MEF conditioned media]])
*Transfer 3 vials of P8 InsACGGsAlov3 from -80 to N2(l) tank.  
*Transfer 3 vials of P8 InsACGGsAlov3 from -80 to N2(l) tank.  
*Transfer all InsACGGsAlov3 c3lls into a new box (named "ZhangLab Box 3). Update cell storage information.
*Transfer all InsACGGsAlov3 c3lls into a new box (named "ZhangLab Box 3). Update cell storage information.
*Transfer LIF from -80 to -20.
*Transfer LIF from -80 to -20.

Latest revision as of 22:46, 4 June 2012

  • Collect MEF conditioned medium (2*10-2=18 mL), store at -20. Add new mESC medium to MEF cells (including the one in T75 flask thawed yesterday).
  • Passage one well of 1:12 P8 InsACGGsAlov3 mESCs into 1:6 (1 well) and 1:3 (1 well; on 0.1% gelatin coating plate without MEF feeder cells; add MEF conditioned media)
  • Transfer 3 vials of P8 InsACGGsAlov3 from -80 to N2(l) tank.
  • Transfer all InsACGGsAlov3 c3lls into a new box (named "ZhangLab Box 3). Update cell storage information.
  • Transfer LIF from -80 to -20.