Tina:LabNotes/2012-5-25: Difference between revisions

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**Passage one well of 1:12 P6 InsACGGsAlov3 into 1:6 (1 well), 1:12 (1 well) and 1:24 (1 well)
*Passage one well of 1:12 P6 InsACGGsAlov3 into 1:6 (1 well), 1:12 (1 well) and 1:24 (1 well)
**Frozen down 3 vials of P6 InsACGGsAlov3 (from one 1:12 well and the remaining part of the passaged 1:12 well). Put cryovials at -80.
*Frozen down 3 vials of P6 InsACGGsAlov3 (from one 1:12 well and the remaining part of the passaged 1:12 well). Put cryovials at -80.
**[[Preparation of 0.1% gelatin solution]]
*[[Preparation of 0.1% gelatin solution]]
**[[Preparation of gelatin coated plates]]  
*[[Preparation of gelatin coated plates]]  
**Seed MEF on gelatin coating plates (using [[MEF culture medium]])
*Seed MEF on gelatin coating plates (using [[MEF culture medium]])

Revision as of 22:54, 4 June 2012