Arichard:Samples/MDA microarray samples: Difference between revisions

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>Andrew
(Created page with "==MDA microarray== * All nuclei from Alzheimer's patient: CTX+ (Neuronal cortex), CTX- (Non-neuronal cortex), CBL+ (Neuronal cerebellum), and CBL- (Neuronal cerebellum). ** 2...")
 
>Andrew
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** Control brain nuclei: 60831 FITC+ (Neuronal) and 60831 FITC- (Non-neuronal). These samples need to be diluted 1:10 in PBS (e.g., 5 ul:45 ul).
** Control brain nuclei: 60831 FITC+ (Neuronal) and 60831 FITC- (Non-neuronal). These samples need to be diluted 1:10 in PBS (e.g., 5 ul:45 ul).
** Cell-free, metaphase chromosomes: GM20431 at 150 parts/ul. Optimal dilution for this sample has not been determined, so we are currently doing both 1:1 (meaning no dilution) and 1:5, both 50 ul total volume.
** Cell-free, metaphase chromosomes: GM20431 at 150 parts/ul. Optimal dilution for this sample has not been determined, so we are currently doing both 1:1 (meaning no dilution) and 1:5, both 50 ul total volume.
* Eukaryotic samples are stored at 4deg C. Prokaryotic samples are stored at -20deg C as glycerol stocks.

Revision as of 20:38, 1 October 2012

MDA microarray

  • All nuclei from Alzheimer's patient: CTX+ (Neuronal cortex), CTX- (Non-neuronal cortex), CBL+ (Neuronal cerebellum), and CBL- (Neuronal cerebellum).
    • 2401 CTX+ needs no dilution.
    • 2401 CBL+ needs 1:10 dilution.
    • Jeff didn't say what dilutions are needed for 2401 CTX- and 2401 CBL-.
  • Other samples are as follows
    • Two types of sea bacteria: Synechococcus and prochlorococcus. These samples are already at the right concentrations, 10-20 parts/ul.
    • Skin bacteria sample from microbiome project. Heterogeneous. I need to ask Jeff about these dilutions.
    • Control brain nuclei: 60831 FITC+ (Neuronal) and 60831 FITC- (Non-neuronal). These samples need to be diluted 1:10 in PBS (e.g., 5 ul:45 ul).
    • Cell-free, metaphase chromosomes: GM20431 at 150 parts/ul. Optimal dilution for this sample has not been determined, so we are currently doing both 1:1 (meaning no dilution) and 1:5, both 50 ul total volume.
  • Eukaryotic samples are stored at 4deg C. Prokaryotic samples are stored at -20deg C as glycerol stocks.