Arichard:Samples/MDA microarray samples: Difference between revisions
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>Andrew No edit summary |
>Andrew No edit summary |
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{| border="1" | {| border="1" | ||
|+ Samples and dilutions | |+ Samples and dilutions | ||
| Skn || 1:1 | | Skn || 1:1 | ||
|- | |- | ||
| Sea || 1:1 | | Sea || 1:1 | ||
|- | |- | ||
| CtlCtx+ || 1:10 | | CtlCtx+ || 1:10 | ||
|- | |- | ||
| CtlCtx- || 1:10 | | CtlCtx- || 1:10 | ||
|- | |- | ||
| AlzCtx+ || 1:1 | | AlzCtx+ || 1:1 | ||
|- | |- | ||
|AlzCtx- || 1:10 | |AlzCtx- || 1:10 | ||
|- | |- | ||
|AlzCbl+ || 1:20 | |AlzCbl+ || 1:20 | ||
|- | |- | ||
|AlzCbl- || 1:10 | |AlzCbl- || 1:10 | ||
|- | |- | ||
| DowCtx+ || 1:10 | | DowCtx+ || 1:10 | ||
|- | |- | ||
| DowCtx || 1:20 | | DowCtx || 1:20 | ||
|} | |} |
Revision as of 20:26, 10 October 2012
MDA microarray
September 13
- All nuclei from Alzheimer's patient: CTX+ (Neuronal cortex), CTX- (Non-neuronal cortex), CBL+ (Neuronal cerebellum), and CBL- (Neuronal cerebellum).
- 2401 CTX+ needs no dilution.
- 2401 CBL+ needs 1:10 dilution.
- Jeff didn't say what dilutions are needed for 2401 CTX- and 2401 CBL-.
- Other samples are as follows
- Two types of sea bacteria: Synechococcus and prochlorococcus. These samples are already at the right concentrations, 10-20 parts/ul.
- Skin bacteria sample from microbiome project. Heterogeneous. I need to ask Jeff about these dilutions.
- Control brain nuclei: 60831 FITC+ (Neuronal) and 60831 FITC- (Non-neuronal). These samples need to be diluted 1:10 in PBS (e.g., 5 ul:45 ul).
- Cell-free, metaphase chromosomes: GM20431 at 150 parts/ul. Optimal dilution for this sample has not been determined, so we are currently doing both 1:1 (meaning no dilution) and 1:5, both 50 ul total volume.
- Eukaryotic samples are stored at 4deg C. Prokaryotic samples are stored at -20deg C as glycerol stocks.
October 10
- Below is the current list of all the samples and required dilutions.
Skn | 1:1 |
Sea | 1:1 |
CtlCtx+ | 1:10 |
CtlCtx- | 1:10 |
AlzCtx+ | 1:1 |
AlzCtx- | 1:10 |
AlzCbl+ | 1:20 |
AlzCbl- | 1:10 |
DowCtx+ | 1:10 |
DowCtx | 1:20 |