Leica SP8 Confocal Microscope: Difference between revisions

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===Leica support contact===
*Technical support: (Toll Free) 866-830-0735
*Clark Thom
West Coast Team Leader Field Service
619-278-8324
clark.thom@leica-microsystems.com
*Carlos Alonso
Confocal Applications Specialist
858-353-8062
carlos.alonso@leica-microsystems.com




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#*1 pixel = 141.92nm @10x obj., 22.53nm @ 63x obj.
#*1 pixel = 141.92nm @10x obj., 22.53nm @ 63x obj.
#*Max. Scan field : 21.2mm (Tile scanning)
#*Max. Scan field : 21.2mm (Tile scanning)
===Sample preparation===
*Only one important consideration is that substrate must be '''#1.5 glass slide'''!
===Starting and Finishing sequence===

Revision as of 22:36, 16 January 2013

Location

PFBH 036

File:Confocal01.jpg File:Confocal02.jpg

Leica support contact

  • Technical support: (Toll Free) 866-830-0735
  • Clark Thom

West Coast Team Leader Field Service 619-278-8324 clark.thom@leica-microsystems.com

  • Carlos Alonso

Confocal Applications Specialist 858-353-8062 carlos.alonso@leica-microsystems.com


General specification

  1. 3 Laser source – 488, 552, 638nm, Solid State, up to 20mW
  2. 2 PMT (Photo Multiplier Tube)
  3. 3 Objective Lens
    • 10x NA = 0.3
    • 20x NA = 0.75(Glycerine or Oil immersion)
    • 63x NA=1.4(Oil immersion)
  4. Z-axis step size – 50nm
  5. Scan area - 1.16 mm x 1.16mm (@10x obj., zoom = 1x) ~ 24.22 um x 24.22um (@10x obj., zoom = 48x)
  6. Image size - up to 8192 x 8192 pixel
    • 1 pixel = 141.92nm @10x obj., 22.53nm @ 63x obj.
    • Max. Scan field : 21.2mm (Tile scanning)


Sample preparation

  • Only one important consideration is that substrate must be #1.5 glass slide!

Starting and Finishing sequence