Leica SP8 Confocal Microscope: Difference between revisions

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===Leica support contact===
===Leica support contact===
#Technical support: (Toll Free) 866-830-0735
#Technical support: (Toll Free) 866-830-0735
#Clark Thom
#Clark Thom
#*West Coast Team Leader Field Service
#*West Coast Team Leader Field Service
#*619-278-8324
#*619-278-8324
#*clark.thom@leica-microsystems.com
#*clark.thom@leica-microsystems.com
#Carlos Alonso
#Carlos Alonso
#*Confocal Applications Specialist
#*Confocal Applications Specialist
Line 47: Line 45:
#*Confocal Power supply : '''Turn on from left to right''' Scan power --> Laser power --> Key (turn clockwise)
#*Confocal Power supply : '''Turn on from left to right''' Scan power --> Laser power --> Key (turn clockwise)
#*PC power strip : Turn on the switch on the strip
#*PC power strip : Turn on the switch on the strip
#Turn off sequence --> Reverse way of turn on sequence
#Turn off sequence --> Reverse way of turn on sequence
#*Shut down PC
#*Shut down PC
#*Turn off PC power strip
#*Turn off PC power strip
#*Turn off confocal power supply : '''Turn off from right to left''' : Key --> Laser power --> Scan power  
#*Turn off confocal power supply : '''Turn off from right to left''' : Key --> Laser power --> Scan power  


{| {{table}}
{| {{table}}
| [[File:ConfocalPower_TurnOn.jpg|200px]]
| [[File:ConfocalPower_TurnOn.jpg|250px]]
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||
| [[File:ConfocalPower_TurnOff.jpg|200px]]
| [[File:ConfocalPower_TurnOff.jpg|250px]]
|}
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Revision as of 22:57, 16 January 2013

Location

PFBH 036

File:Confocal01.jpg File:Confocal02.jpg


Leica support contact

  1. Technical support: (Toll Free) 866-830-0735
  2. Clark Thom
    • West Coast Team Leader Field Service
    • 619-278-8324
    • clark.thom@leica-microsystems.com
  3. Carlos Alonso
    • Confocal Applications Specialist
    • 858-353-8062
    • carlos.alonso@leica-microsystems.com
  • (Call technical support first before trying to contact Clark)


General specification

  1. 3 Laser source – 488, 552, 638nm, Solid State, up to 20mW
  2. 2 PMT (Photo Multiplier Tube)
  3. 3 Objective Lens
    • 10x NA = 0.3
    • 20x NA = 0.75(Glycerine or Oil immersion)
    • 63x NA=1.4(Oil immersion)
  4. Z-axis step size – 50nm
  5. Scan area - 1.16 mm x 1.16mm (@10x obj., zoom = 1x) ~ 24.22 um x 24.22um (@10x obj., zoom = 48x)
  6. Image size - up to 8192 x 8192 pixel
    • 1 pixel = 141.92nm @10x obj., 22.53nm @ 63x obj.
    • Max. Scan field : 21.2mm (Tile scanning)


Sample preparation

  • Only one important consideration is that substrate must be #1.5 glass slide!


Turning on/off sequence

  1. Turn on sequence
    • Confocal Power supply : Turn on from left to right Scan power --> Laser power --> Key (turn clockwise)
    • PC power strip : Turn on the switch on the strip
  2. Turn off sequence --> Reverse way of turn on sequence
    • Shut down PC
    • Turn off PC power strip
    • Turn off confocal power supply : Turn off from right to left : Key --> Laser power --> Scan power


File:ConfocalPower TurnOn.jpg File:ConfocalPower TurnOff.jpg