Hosuk:LabNotesMDA/2013-2-5: Difference between revisions
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>Hosuki78 (Created page with "====qPCR run==== *Run qPCR with the sample from 1/28, 1/29 and 2/4 using mouse and human primer *The number of tubes **1/28: 16ea (#9~#24) **1/29: 21ea (#1~#21) **2/4: 23ea (...") |
>Hosuki78 No edit summary |
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====Result==== | ====Result==== | ||
* | *Most of curves rose after 24 cycle, and became flat around 32 cycle | ||
*Seemed too much low amount of MDA product | |||
*Human DNA has also been seen. | |||
*(need more description) | |||
[[File:qPCR_curve_2013-02-05.jpg|300px]] |
Revision as of 17:36, 6 February 2013
qPCR run
- Run qPCR with the sample from 1/28, 1/29 and 2/4 using mouse and human primer
- The number of tubes
- 1/28: 16ea (#9~#24)
- 1/29: 21ea (#1~#21)
- 2/4: 23ea (#1~#24)
- Preparation
- 1/28 samples: 8 tubes by taking 0.5uL from two tubes and merging into one sample
- 1/28 samples: 8 tubes by taking 0.5uL from two tubes and merging into one sample
- PCR master mix
- KAPA fast
- KAPA fast 15 uL
- 10 uM primer 1 .6 uL
- 10 uM primer 2 .6 uL
- DNA from library 2 uL
- water 11.4 uL
- total 30 uL
- KAPA fast
PCR reaction
- 95C 10sec
- 58C 20sec
- 72C 45sec
- 40cycle
Result
- Most of curves rose after 24 cycle, and became flat around 32 cycle
- Seemed too much low amount of MDA product
- Human DNA has also been seen.
- (need more description)