Jie:LabNotes/oligo sequences: Difference between revisions
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20080716 | 20080716 | ||
primer | primer to produce spacer:refer to LabNotes on [http://genome-tech.ucsd.edu/LabNotes/index.php/Jie:LabNotes/ExonomeSeq/2008-7-16] | ||
CirclehelperV4_short(20080825): | CirclehelperV4_short(20080825): | ||
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20080904 | 20080904 | ||
Prmer used to amplify the cpg30k and cosmic3760. The amplicon will have 5-terminal phosphate, thus can be ligated after circlehelper insertion. | |||
AmpFV6.2_2: 5Phos/C*T*GCAGATGTTATCGAGGTC | AmpFV6.2_2: 5Phos/C*T*GCAGATGTTATCGAGGTC | ||
AmpRV6.2_2: 5Phos/CTGAATAGGAACGATGAGCC | AmpRV6.2_2: 5Phos/CTGAATAGGAACGATGAGCC | ||
20080918 | 20080918 | ||
confirmation primers for cpg30k. Primers targeting bisulfite conversed DNA to confirm the capture of cpg30K | confirmation primers for cpg30k. Primers targeting bisulfite conversed DNA to confirm the capture results of cpg30K. | ||
[[Media: 20080918_conirmed primers for cpg30k.xls]] | [[Media: 20080918_conirmed primers for cpg30k.xls]] |
Revision as of 17:31, 19 September 2008
20080716
primer to produce spacer:refer to LabNotes on [1]
CirclehelperV4_short(20080825):
AACAGTGCTCTTCCAGTCTACTAGCCTCATGCGTATCCGATC
20080904
Prmer used to amplify the cpg30k and cosmic3760. The amplicon will have 5-terminal phosphate, thus can be ligated after circlehelper insertion. AmpFV6.2_2: 5Phos/C*T*GCAGATGTTATCGAGGTC AmpRV6.2_2: 5Phos/CTGAATAGGAACGATGAGCC
20080918
confirmation primers for cpg30k. Primers targeting bisulfite conversed DNA to confirm the capture results of cpg30K. Media: 20080918_conirmed primers for cpg30k.xls