Jie:LabNotes/oligo sequences: Difference between revisions

From ZhangLabWiki
Jump to navigation Jump to search
>Jie deng
No edit summary
>Jie deng
No edit summary
Line 1: Line 1:
20080716
20080716
   primer for spacer:refer to LabNotes on [http://genome-tech.ucsd.edu/LabNotes/index.php/Jie:LabNotes/ExonomeSeq/2008-7-16]
   primer to produce spacer:refer to LabNotes on [http://genome-tech.ucsd.edu/LabNotes/index.php/Jie:LabNotes/ExonomeSeq/2008-7-16]


CirclehelperV4_short(20080825):
CirclehelperV4_short(20080825):
Line 6: Line 6:


20080904
20080904
  Prmer used to amplify the cpg30k and cosmic3760. The amplicon will have 5-terminal phosphate, thus can be ligated after circlehelper insertion.
   AmpFV6.2_2: 5Phos/C*T*GCAGATGTTATCGAGGTC
   AmpFV6.2_2: 5Phos/C*T*GCAGATGTTATCGAGGTC
   AmpRV6.2_2: 5Phos/CTGAATAGGAACGATGAGCC
   AmpRV6.2_2: 5Phos/CTGAATAGGAACGATGAGCC


20080918
20080918
   confirmation primers for cpg30k. Primers targeting bisulfite conversed DNA to confirm the capture of cpg30K is unbiased.
   confirmation primers for cpg30k. Primers targeting bisulfite conversed DNA to confirm the capture results of cpg30K.
   [[Media: 20080918_conirmed primers for cpg30k.xls]]
   [[Media: 20080918_conirmed primers for cpg30k.xls]]

Revision as of 17:31, 19 September 2008

20080716

 primer to produce spacer:refer to LabNotes on [1]

CirclehelperV4_short(20080825):

 AACAGTGCTCTTCCAGTCTACTAGCCTCATGCGTATCCGATC

20080904

 Prmer used to amplify the cpg30k and cosmic3760. The amplicon will have 5-terminal phosphate, thus can be ligated after circlehelper insertion.
 AmpFV6.2_2: 5Phos/C*T*GCAGATGTTATCGAGGTC
 AmpRV6.2_2: 5Phos/CTGAATAGGAACGATGAGCC

20080918

 confirmation primers for cpg30k. Primers targeting bisulfite conversed DNA to confirm the capture results of cpg30K.
 Media: 20080918_conirmed primers for cpg30k.xls