Noi/NOTES/2013-2-27: Difference between revisions
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>Noi (Created page with "* http://genome-tech.ucsd.edu/LabNotes/index.php/noi:DMR220k_LabNotes '''Link to calendar''' == Plan for probe preparation for Matt's project == * I will be preparing prob...") |
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'''Probe prep''' | '''Probe prep''' | ||
- Waiting for seed oligo: expected to come next Wednesday (March6, 2013) | - Waiting for seed oligo: expected to come next Wednesday (March6, 2013) | ||
- eMIP_CA1_F and eMIP_CA1_R primer for expansion PCR and production PCR | - eMIP_CA1_F and eMIP_CA1_R primer for expansion PCR and production PCR (very little left, ordered 2/28/2013) | ||
- 2x KAPA SYBG FAST qPCR MM | - 2x KAPA SYBG FAST qPCR MM | ||
- Nt.AlwI (NEB) and Nb.BsrDI (NEB) -> ordered | - Nt.AlwI (NEB) and Nb.BsrDI (NEB) -> ordered 2/26/2013 | ||
- Qiaquick colums, TBU gel, Nanosep column | - Qiaquick colums, TBU gel, Nanosep column | ||
'''Padlock capture''' | '''Padlock capture''' | ||
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- Ampligase and buffer | - Ampligase and buffer | ||
- ExoI/ExoIII | - ExoI/ExoIII | ||
- Amplification | - Amplification primers (Matt) |
Revision as of 19:28, 28 February 2013
Plan for probe preparation for Matt's project
- I will be preparing probes using the same method as I did for LC Sciences oligoes, [LC_Sciences probe prep]
- Check reagents to make sure I have all reagents ready for probe prep and padlock capture
Probe prep - Waiting for seed oligo: expected to come next Wednesday (March6, 2013) - eMIP_CA1_F and eMIP_CA1_R primer for expansion PCR and production PCR (very little left, ordered 2/28/2013) - 2x KAPA SYBG FAST qPCR MM - Nt.AlwI (NEB) and Nb.BsrDI (NEB) -> ordered 2/26/2013 - Qiaquick colums, TBU gel, Nanosep column Padlock capture Note: There is no gap for this capture, so only need Ampligase - Ampligase and buffer - ExoI/ExoIII - Amplification primers (Matt)