Noi/NOTES/2013-2-27: Difference between revisions

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* Check reagents to make sure I have all reagents ready for probe prep and padlock capture
* Check reagents to make sure I have all reagents ready for probe prep and padlock capture
  '''Probe prep'''
  '''Probe prep'''
  - Waiting for seed oligo: expected to come next Wednesday (March6, 2013)
  - Waiting for seed oligos: expected to come next Wednesday (March6, 2013)
  - eMIP_CA1_F and eMIP_CA1_R primer for expansion PCR and production PCR (very little left, ordered 2/28/2013)
  - eMIP_CA1_F and eMIP_CA1_R primer for expansion PCR and production PCR (very little left, ordered 2/28/2013)
  - 2x KAPA SYBG FAST qPCR MM
  - 2x KAPA SYBG FAST qPCR MM
  - Nt.AlwI (NEB) and Nb.BsrDI (NEB) -> ordered 2/26/2013
  - Nt.AlwI (NEB) and Nb.BsrDI (NEB) -> ordered 2/26/2013
  - Qiaquick colums, TBU gel, Nanosep column
  - Qiaquick columns, TBU gel, Nanosep columns
  '''Padlock capture'''
  '''Padlock capture'''
  Note: There is no gap for this capture, so only need Ampligase
  Note: There is no gap for this capture, so only need Ampligase

Revision as of 00:14, 1 March 2013

Plan for probe preparation for Matt's project

  • I will be preparing probes using the same method as I did for LC Sciences oligoes, [[1]]
  • Check reagents to make sure I have all reagents ready for probe prep and padlock capture
Probe prep
- Waiting for seed oligos: expected to come next Wednesday (March6, 2013)
- eMIP_CA1_F and eMIP_CA1_R primer for expansion PCR and production PCR (very little left, ordered 2/28/2013)
- 2x KAPA SYBG FAST qPCR MM
- Nt.AlwI (NEB) and Nb.BsrDI (NEB) -> ordered 2/26/2013
- Qiaquick columns, TBU gel, Nanosep columns
Padlock capture
Note: There is no gap for this capture, so only need Ampligase
- Ampligase and buffer
- ExoI/ExoIII
- Amplification primers (Matt)