Blue:RNA-Seq Experiments:03212013: Difference between revisions
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Purpose: Test RNAseIII single cell totoRNAseq protocol - Same as for 03192013 except with a longer RNAseIII incubation time (increased from 10min to 20min) | |||
Result: Only minor downward shift in library fragment size - still too many high molecular weight fragments and poor amplification after DSN treatment | |||
[[File:03212013.pdf]] | [[File:03212013.pdf]] |
Latest revision as of 22:11, 25 March 2013
Purpose: Test RNAseIII single cell totoRNAseq protocol - Same as for 03192013 except with a longer RNAseIII incubation time (increased from 10min to 20min)
Result: Only minor downward shift in library fragment size - still too many high molecular weight fragments and poor amplification after DSN treatment