Daniel:Notebook/HiResChrPaint/2013-4-15: Difference between revisions

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===Dye Coupling Reaction===
===Dye Coupling Reaction===
   Thaw out oligos, sodium bicarbonate solution, DMSO
   1. Thaw out oligos, sodium bicarbonate solution, DMSO
     Use thermo device on Noi's desk to heat DMSO, sodium bicarb to 42 C
     Use thermo device on Noi's desk to heat DMSO, sodium bicarb to 42 C
   Add 2.5 uL DNA, 1.5 uL sodium bicarbonate to 0.2 mL eppendorf
   2. Add 2.5 uL DNA, 1.5 uL sodium bicarbonate to 0.2 mL eppendorf
   Thaw out dyes (they are in big -20C) and resuspend in 2.2 uL DMSO
   3. Thaw out dyes (they are in big -20C) and resuspend in 2.2 uL DMSO
   Add 1 uL of appropriate dye to oligo set (see below)
    Use dyes immediately, they do not store
   4. Add 1 uL of appropriate dye to oligo set (see below)


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Revision as of 18:15, 15 April 2013

Dye Coupling Test and Gel Quantification

 Purpose:  Test dye coupling reaction, specifically the purification step, to ensure proper dye-coupled DNA is being captured.
 Two samples chosen from Gel Quantification (4/10/13). 
 Oligos coupled to appropriate dye.

Dye Coupling Reaction

 1. Thaw out oligos, sodium bicarbonate solution, DMSO
    Use thermo device on Noi's desk to heat DMSO, sodium bicarb to 42 C
 2. Add 2.5 uL DNA, 1.5 uL sodium bicarbonate to 0.2 mL eppendorf
 3. Thaw out dyes (they are in big -20C) and resuspend in 2.2 uL DMSO
    Use dyes immediately, they do not store
 4. Add 1 uL of appropriate dye to oligo set (see below)

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