Daniel:Notebook/HiResChrPaint/2013-4-15: Difference between revisions

From ZhangLabWiki
Jump to navigation Jump to search
>Djacobse
No edit summary
>Djacobse
No edit summary
Line 11: Line 11:
     Use dyes immediately, they do not store
     Use dyes immediately, they do not store
   4. Add 1 uL of appropriate dye to oligo set (see below)
   4. Add 1 uL of appropriate dye to oligo set (see below)
{| class="wikitable" <hiddentext>generated with [[:de:Wikipedia:Helferlein/VBA-Macro for EXCEL tableconversion]] V1.8</hiddentext>
|- style="font-size:12pt" align="center"
| width="65" height="16" | Probe
| width="65" | Alexa Dye


|- style="font-size:12pt" align="center"
| height="16" | V4S1
| align="right" | 488
|- style="font-size:12pt" align="center"
| height="16" | V4S2
| align="right" | 546
|- style="font-size:12pt" align="center"
| height="16" | V4S3
| align="right" | 594
|- style="font-size:12pt" align="center"
| height="16" | V4S4
| align="right" | 647
|- style="font-size:12pt" align="center"
| height="16" | V6S1
| align="right" | 488
|- style="font-size:12pt" align="center"
| height="16" | V6S2
| align="right" | 546
|- style="font-size:12pt" align="center"
| height="16" | V6S3
| align="right" | 594
|- style="font-size:12pt" align="center"
| height="16" | V6S4
| align="right" | 647
|}
[[Daniel:Notebook/HiResChrPaint|Back to Calendar]]
[[Daniel:Notebook/HiResChrPaint|Back to Calendar]]

Revision as of 18:17, 15 April 2013

Dye Coupling Test and Gel Quantification

 Purpose:  Test dye coupling reaction, specifically the purification step, to ensure proper dye-coupled DNA is being captured.
 Two samples chosen from Gel Quantification (4/10/13). 
 Oligos coupled to appropriate dye.

Dye Coupling Reaction

 1. Thaw out oligos, sodium bicarbonate solution, DMSO
    Use thermo device on Noi's desk to heat DMSO, sodium bicarb to 42 C
 2. Add 2.5 uL DNA, 1.5 uL sodium bicarbonate to 0.2 mL eppendorf
 3. Thaw out dyes (they are in big -20C) and resuspend in 2.2 uL DMSO
    Use dyes immediately, they do not store
 4. Add 1 uL of appropriate dye to oligo set (see below)
Probe Alexa Dye
V4S1 488
V4S2 546
V4S3 594
V4S4 647
V6S1 488
V6S2 546
V6S3 594
V6S4 647

Back to Calendar