Daniel:Notebook/HiResChrPaint/2013-3-27: Difference between revisions

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(Created page with "=Probe Set Prep (Started 3/26/2013)= Back to Calendar ==Expansion PCR and Ethanol Precipitation==")
 
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[[Daniel:Notebook/HiResChrPaint|Back to Calendar]]
[[Daniel:Notebook/HiResChrPaint|Back to Calendar]]


==Expansion PCR and Ethanol Precipitation==
==Ethanol Precipitation ([[Daniel:Notebook/HiResChrPaint/2013-3-26|From 3/26/2013]])==
 
  4. Centrifuge at 3000 rpm for 30 min, 4 C
  5. Remove Supernatant and add 600 uL chilled 75% EtOH
  6. Transfer DNA pellet to a 1.5 mL tube
  7. Spin at 14000 rpm for 5 min
  8. Dry the pellet in the hood and resuspend with 100 uL ddH2O
  9. Run 2 Qiaquick columns per sample, elute with 40 uL for each column

Revision as of 23:08, 17 April 2013

Probe Set Prep (Started 3/26/2013)

Back to Calendar

Ethanol Precipitation (From 3/26/2013)

 4. Centrifuge at 3000 rpm for 30 min, 4 C
 5. Remove Supernatant and add 600 uL chilled 75% EtOH
 6. Transfer DNA pellet to a 1.5 mL tube
 7. Spin at 14000 rpm for 5 min
 8. Dry the pellet in the hood and resuspend with 100 uL ddH2O
 9. Run 2 Qiaquick columns per sample, elute with 40 uL for each column