Daniel:Notebook/HiResChrPaint/2013-3-27: Difference between revisions

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   7. Spin at 14000 rpm for 5 min
   7. Spin at 14000 rpm for 5 min
   8. Dry the pellet in the hood and resuspend with 100 uL ddH2O
   8. Dry the pellet in the hood and resuspend with 100 uL ddH2O
   9. Run 2 Qiaquick columns per sample, elute with 40 uL for each column
   9. Run 2 [[Daniel:Protocols/Qiaquick_Column|Qiaquick columns]] per sample, elute with 40 uL for each column
   10. Pool samples (16 columns -> 8 samples)
   10. Pool samples (16 columns -> 8 samples)
   11. Measure concentration with nanodrop
   11. Measure concentration with nanodrop

Latest revision as of 23:44, 17 April 2013

Probe Set Prep (Started 3/26/2013)[edit]

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Ethanol Precipitation (From 3/26/2013)[edit]

 4. Centrifuge at 3000 rpm for 30 min, 4 C
 5. Remove Supernatant and add 600 uL chilled 75% EtOH
 6. Transfer DNA pellet to a 1.5 mL tube
 7. Spin at 14000 rpm for 5 min
 8. Dry the pellet in the hood and resuspend with 100 uL ddH2O
 9. Run 2 Qiaquick columns per sample, elute with 40 uL for each column
 10. Pool samples (16 columns -> 8 samples)
 11. Measure concentration with nanodrop
Probe ng/uL uL ng
V4S1 36.4 80 2912
V4S2 139.7 80 11176
V4S3 76.2 80 6096
V4S4 47.8 80 3824
V6S1 84.4 80 6752
V6S2 111.9 80 8952
V6S3 131.4 80 10512
V6S4 106.9 80 8552