Daniel:Protocols/Quiaquick Column: Difference between revisions

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=Qiaquick Column Purification=
=Qiaquick Column Purification=
Qiagen: Catalog #28104 (50) or #28106 (250)
==Protocol==
  1. Add 5X PB (binding buffer) to sample
  2. Load sample onto column and spin for 1 minute at 14000 rpm; discard flow through
  3. Add 750 uL PE (wash buffer) to column and spin for 1 minute at 14000 rpm; discard flow through
  4. Dry spin column for 1 minute at 14000 rpm; discard flow through
  5. Let stand with cover open in fume hood for ~5 minutes
  6. Transfer column to a new 1.5 mL eppendorf tube
  7. Add ≥30 uL EB to column
  8. Let stand 1 minute
  9. Spin for 1 minute at 14000 rpm




[[Daniel Jacobsen|Back to Main]]
[[Daniel Jacobsen|Back to Main]]

Revision as of 23:42, 17 April 2013

Qiaquick Column Purification

Qiagen: Catalog #28104 (50) or #28106 (250)

Protocol

 1. Add 5X PB (binding buffer) to sample
 2. Load sample onto column and spin for 1 minute at 14000 rpm; discard flow through
 3. Add 750 uL PE (wash buffer) to column and spin for 1 minute at 14000 rpm; discard flow through
 4. Dry spin column for 1 minute at 14000 rpm; discard flow through
 5. Let stand with cover open in fume hood for ~5 minutes
 6. Transfer column to a new 1.5 mL eppendorf tube
 7. Add ≥30 uL EB to column
 8. Let stand 1 minute
 9. Spin for 1 minute at 14000 rpm


Back to Main